![Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Western-Blot-NBP2-37513-img0003.jpg "Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC, CyTOF-ready |
| Clone | 4G4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human Raf-1 expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RAF1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 73 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Raf-1 Antibody (NBP2-37513)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RAF1 |
![Immunohistochemistry: Raf-1 Antibody (4G4) [NBP2-37513] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAF1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Immunohistochemistry-NBP2-37513-img0005.jpg "Immunohistochemistry: Raf-1 Antibody (4G4) [NBP2-37513] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAF1 mouse mAb with DAB staining.")
![Flow Cytometry: Raf-1 Antibody (4G4) [NBP2-37513] - Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Flow-Cytometry-NBP2-37513-img0004.jpg "Flow Cytometry: Raf-1 Antibody (4G4) [NBP2-37513] - Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and negative control (red).")
![Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recombinant protein. (Expected MW is 48.6 kDa)](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-Western-Blot-NBP2-37513-img0002.jpg "Western Blot: Raf-1 Antibody (4G4) [NBP2-37513] - Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recombinant protein. (Expected MW is 48.6 kDa)")
![ELISA: Raf-1 Antibody (4G4) [NBP2-37513] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Raf-1-Antibody-4G4-ELISA-NBP2-37513-img0001.jpg "ELISA: Raf-1 Antibody (4G4) [NBP2-37513] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")