![Western Blot: Rad17 [p Ser656] Antibody [NBP3-13072] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500.](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Western-Blot-NBP3-13072-img0001.jpg "Western Blot: Rad17 [p Ser656] Antibody [NBP3-13072] - Untreated (-) and treated (+) 293T whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide surrounding phospho Ser656 of human Rad17. The exact sequence is proprietary. |
| Modification | p Ser656 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | RAD17 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Rad17 Antibody (NBP3-13072)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RAD17 |
![Immunocytochemistry/Immunofluorescence: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad17 (phospho Ser656) protein stained by Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13072-img0004.jpg "Immunocytochemistry/Immunofluorescence: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad17 (phospho Ser656) protein stained by Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Immunohistochemistry-Paraffin-NBP3-13072-img0003.jpg "Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Rad17-[p-Ser656]-Antibody-Immunohistochemistry-Paraffin-NBP3-13072-img0002.jpg "Immunohistochemistry-Paraffin: Rad17 [p Ser656] Antibody [NBP3-13072] - Rad17 (phospho Ser656) antibody detects Rad17 (phospho Ser656) protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Rad17 (phospho Ser656) antibody (NBP3-13072) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")