![Western Blot: CHTF18 Antibody (1F5) [H00063922-M01] - CHTF18 monoclonal antibody (M01), clone 1F5 Analysis of CHTF18 expression in HeLa.](http://images.novusbio.com/fullsize/CHTF18-Antibody-1F5-Western-Blot-H00063922-M01-img0005.jpg "Western Blot: CHTF18 Antibody (1F5) [H00063922-M01] - CHTF18 monoclonal antibody (M01), clone 1F5 Analysis of CHTF18 expression in HeLa.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | 1F5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | CHTF18 (AAH18184, 886 a.a. ~ 975 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVHRPAPRNHEQRLEHIMRRAAREEQPEKDFFGRVVVRSTAVPSAGDTAPEQDSVERRMGTAVGRSEVWFRFNEGVSNAVRRSLYIRDLL |
| Specificity | CHTF18 - CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CHTF18 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 22493068) |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
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![Western Blot: CHTF18 Antibody (1F5) [H00063922-M01] - Analysis of CHTF18 expression in transfected 293T cell line by CHTF18 monoclonal antibody (M01), clone 1F5.Lane 1: CHTF18 transfected lysate(107 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CHTF18-Antibody-1F5-Western-Blot-H00063922-M01-img0006.jpg "Western Blot: CHTF18 Antibody (1F5) [H00063922-M01] - Analysis of CHTF18 expression in transfected 293T cell line by CHTF18 monoclonal antibody (M01), clone 1F5.Lane 1: CHTF18 transfected lysate(107 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: CHTF18 Antibody (1F5) [H00063922-M01] - Detection limit for recombinant GST tagged CHTF18 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CHTF18-Antibody-1F5-Sandwich-ELISA-H00063922-M01-img0004.jpg "Sandwich ELISA: CHTF18 Antibody (1F5) [H00063922-M01] - Detection limit for recombinant GST tagged CHTF18 is approximately 0.1ng/ml as a capture antibody.")
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