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R-Spondin 1 Antibody (852010) [Unconjugated]

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Recombinant Mouse R-Spondin 1 (7150-RS) co-stimulates beta -catenin response in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line). beta -Catenin Response elicited by Recombinant Mouse R-Spondin 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse R-Spondin 1 Mono-clonal Antibody (Catalog # MAB3474) in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (1324-WN). The ND50 is typically 0.4-2 µg/mL.
Recombinant Mouse R-Spondin 1 (7150-RS) co-stimulates beta -catenin response in the HEK293T human embryonic kidney cell line in a dose-dependent manner (orange line). beta -Catenin Response elicited by Recombinant ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications B/N
Clone
852010
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3474-SP
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R-Spondin 1 Antibody (852010) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse R‑Spondin 1
Met1-Gln265
Accession # Q9Z132
Specificity
Detects recombinant mouse (rm) R‑Spondin 1 in ELISA. In ELISA, no cross-reactivity with rmR-Spondin 3 and rhR-Spondin 1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
RSPO1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
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Applications/Dilutions

Dilutions
  • Neutralization 0.4-2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for R-Spondin 1 Antibody (852010) [Unconjugated]

  • Cristin 3
  • CRISTIN3
  • FLJ40906Roof plate-specific spondin-1
  • HRspo1
  • roof plate-specific spondin
  • RSPO
  • RSPO1
  • RSpondin 1
  • R-Spondin 1
  • R-spondin homolog (Xenopus laevis)
  • RSPONDIN
  • R-spondin1
  • R-spondin-1

Background

RSPO-1 (roof plate-specific spondin; also R-Spondin 1 and cristin3) is a 39 kDa, heparin-binding member of the Cristin/R-Spondin family of proteins. These molecules apparently are either retained intracellularly or secreted, where they activate the frizzled-LRP receptor pathway. Mature human RSPO-1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. A presumably glycosylated, 30 kDa, 193 aa C-terminally truncated form is secreted and interacts with heparin. The secreted form of human RSPO-1 is 93% aa identical to secreted mouse RSPO-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RSPO1
Uniprot