![Western Blot: PUF60 Antibody [NB100-1125] - Staining of A431 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NB100-1125-img0002.jpg "Western Blot: PUF60 Antibody [NB100-1125] - Staining of A431 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-YDQERFDNSDLSA corresponding to C-Terminus according to NP_510965.1, NP_055096.2, NP_001129505.1. |
| Localization | Cytoplasmic |
| Specificity | This antibody is expected to recognise all reported human isoforms (as represented by NP_510965.1; NP_055096.2; NP_001129505.1) |
| Predicted Species | Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | PUF60 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 60 kDa band observed in human lysates of HEK293 and HepG2 (calculated MW of 59.9 kDa band according to NP_510965.1 and 58.2 according to NP_055096.2). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
![Flow Cytometry FOLR1 Antibody (548908) [Unconjugated]](https://images.novusbio.com/images2/FOLR1_MAB5646_Flow_Cytometry_10781.jpg)
![Western Blot FOLR1 Antibody (548908) [Unconjugated]](https://images.novusbio.com/images2/FOLR1_MAB5646_Western_Blot_6248.jpg)
![Immunocytochemistry FOLR1 Antibody (548908) [Unconjugated]](https://images.novusbio.com/images2/FOLR1_MAB5646_Immunocytochemistry_6624.jpg)
| Publication using NB100-1125 | Applications | Species |
|---|---|---|
| Liu J, He L, Collins I et al. The FBP interacting repressor targets TFIIH to inhibit activated transcription. Mol Cell 2000-02-01 [PMID: 10882074] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: PUF60 Antibody [NB100-1125] - Staining of paraffin embedded Human Skin. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NB100-1125-img0005.jpg "Immunohistochemistry-Paraffin: PUF60 Antibody [NB100-1125] - Staining of paraffin embedded Human Skin. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![PUF60 Antibody [NB100-1125] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-NB100-1125-img0004.jpg "PUF60 Antibody [NB100-1125] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
