![Western Blot: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis using PTH1R mAb against PTH1R (AA: 27-188)-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-4D2-Western-Blot-NBP1-51640-img0010.jpg "Western Blot: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis using PTH1R mAb against PTH1R (AA: 27-188)-hIgGFc transfected HEK293 cell lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 4D2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Purified recombinant fragment of human PTH1R/PTHR1 expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PTH1R |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Theoretical MW | 66 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Ascites |
| Preservative | 0.03% Sodium Azide |
| Purity | Unpurified |
![Immunohistochemistry PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Immunohistochemistry_16650.jpg)
![Western Blot PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Western_Blot_16656.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for PTH1R/PTHR1 Antibody (NBP1-51640)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Generation of functional kidney spheroids using immortalized mouse primary cells. Representative images of mKidS immunostained for p-GP, OCT2, and PTHR. All data are shown as the means?+-?s.e.m. *p?less than ?0.05 compared with 2D or 3D control by unpaired Student's t-test. Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-53855-2) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-4D2-Immunocytochemistry-Immunofluorescence-NBP1-51640-img0011.jpg "Immunocytochemistry/Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Generation of functional kidney spheroids using immortalized mouse primary cells. Representative images of mKidS immunostained for p-GP, OCT2, and PTHR. All data are shown as the means?+-?s.e.m. *p?less than ?0.05 compared with 2D or 3D control by unpaired Student's t-test. Scale bar, 20 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-53855-2) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis of human kidney tissues using PTH1R mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-4D2-Immunohistochemistry-Paraffin-NBP1-51640-img0009.jpg "Immunohistochemistry-Paraffin: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis of human kidney tissues using PTH1R mouse mAb with DAB staining.")
![Immunocytochemistry/Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis of SK-BR-3 cells using PTH1R mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/PTH1R-PTHR1-Antibody-4D2-Immunocytochemistry-Immunofluorescence-NBP1-51640-img0007.jpg "Immunocytochemistry/Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) [NBP1-51640] - Analysis of SK-BR-3 cells using PTH1R mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.")
![Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) - BSA Free [NBP1-51640] - Generation of functional kidney spheroids using immortalized human primary cells. (a) Quantitative RT-PCR analysis of kidney-specific segment markers in hKidS compared with 2D controls (2D CTL) & human kidney lysates (hKidney). n = 8 for 2D CTL & hKidS & n = 3 for hKidney. (b) Western blotting analysis for renal multi-lineage marker in mouse kidney spheroids compared to 2D CTL & hKidney. The grouping of gels/blots cropped from different parts of the same gel. (c) Representative images for cell polarity of Zo-1, an apical membrane, & Na/K ATPase, a basolateral membrane marker in hKidS. Scale bar, 10 μm. (d) Representative images of human kidney spheroids (hKidS) at day 5 of maturation stained for the kidney-specific markers LTL, PNA, AQP2, & Nephrin. Human kidney was used as positive control for immunostaining of hKidS. (e) Responses to parathyroid hormone (PTH) of hKidS compared with 3D control spheroids (without maturation factors, CTL) & 2D cells. (f) GGT activity in hKidS compared with 3D CTL. (g) Temperature-dependent albumin-fluorescein uptake of hKidS compared to 3D CTL. n = 10 for 3D CTL & hKidS. (h,i) Relative mRNA expression of apical (h) & basolateral (i) transporters in hKidS compared to 2D cultured cells, hPTECs spheroids generated using the same protocol for hKidS (hPTECs-3D), & hKidney. n = 9 for 2D CTL & hKidS & n = 3 for hPTECs-3D & hKidney. (j) Representative images of hKidS immunostained for p-GP, OCT2, & PTHR. All data are shown as the means ± s.e.m. *p < 0.05 compared with 2D or 3D control by unpaired Student’s t-test. Scale bar, 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31772214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51640_mouse-monoclonal-pth1r-pthr1-antibody-4d2-310202416171477.jpg "Immunocytochemistry/ Immunofluorescence: PTH1R/PTHR1 Antibody (4D2) - BSA Free [NBP1-51640] - Generation of functional kidney spheroids using immortalized human primary cells. (a) Quantitative RT-PCR analysis of kidney-specific segment markers in hKidS compared with 2D controls (2D CTL) & human kidney lysates (hKidney). n = 8 for 2D CTL & hKidS & n = 3 for hKidney. (b) Western blotting analysis for renal multi-lineage marker in mouse kidney spheroids compared to 2D CTL & hKidney. The grouping of gels/blots cropped from different parts of the same gel. (c) Representative images for cell polarity of Zo-1, an apical membrane, & Na/K ATPase, a basolateral membrane marker in hKidS. Scale bar, 10 μm. (d) Representative images of human kidney spheroids (hKidS) at day 5 of maturation stained for the kidney-specific markers LTL, PNA, AQP2, & Nephrin. Human kidney was used as positive control for immunostaining of hKidS. (e) Responses to parathyroid hormone (PTH) of hKidS compared with 3D control spheroids (without maturation factors, CTL) & 2D cells. (f) GGT activity in hKidS compared with 3D CTL. (g) Temperature-dependent albumin-fluorescein uptake of hKidS compared to 3D CTL. n = 10 for 3D CTL & hKidS. (h,i) Relative mRNA expression of apical (h) & basolateral (i) transporters in hKidS compared to 2D cultured cells, hPTECs spheroids generated using the same protocol for hKidS (hPTECs-3D), & hKidney. n = 9 for 2D CTL & hKidS & n = 3 for hPTECs-3D & hKidney. (j) Representative images of hKidS immunostained for p-GP, OCT2, & PTHR. All data are shown as the means ± s.e.m. *p < 0.05 compared with 2D or 3D control by unpaired Student’s t-test. Scale bar, 20 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31772214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry PTHLH/PTHrP Antibody (677939) [Unconjugated]](https://images.novusbio.com/images2/PTHLH_MAB6734_Immunohistochemistry_10816.jpg)
![Flow Cytometry Lgr6 Antibody (918719) [Unconjugated]](https://images.novusbio.com/images2/Lgr6_MAB8458_Flow_Cytometry_16097.jpg)
![Immunocytochemistry Lgr6 Antibody (918719) [Unconjugated]](https://images.novusbio.com/images2/Lgr6_MAB8458_Immunocytochemistry_16415.jpg)
![SDS-PAGE TRANCE/TNFSF11/RANK L [Unconjugated]](https://images.novusbio.com/images2/TRANCE_462-TEC_442.jpg)
![Bioactivity TRANCE/TNFSF11/RANK L [Unconjugated]](https://images.novusbio.com/images2/TRANCE_462-TEC_556.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")