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Canine PTH ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Standard Curve Range
9.88 - 800 pg/mL
Sensitivity
4.38 pg/mL

Order Details

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Canine PTH ELISA Kit (Colorimetric) Summary

Description
Assay time: 2 hours
Standard Curve Range
9.88 - 800 pg/mL
Sensitivity
4.38 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PTH

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30x concentrate)

Alternate Names for Canine PTH ELISA Kit (Colorimetric)

  • Parathormone
  • Parathyrin
  • parathyroid hormone 1
  • parathyroid hormone
  • PTH
  • PTH1

Background

Parathyroid hormone (PTH), which is also designated parathyrin, is an 84 amino acid single chain peptide that functions to regulate calcium metabolism by raising blood levels of calcium through various mechanisms. PTH stimulates bone formation to increase bone mass and strength in rats and humans. Within the PTH molecule, the essential activity is associated with the first 34 amino acids at the amino-terminus of the molecule. The gene encoding PTH maps to human chromosome 11p15.3-p15.1. Parathyroid hormone-related protein (PTH-rP) is an autocrine factor that is structurally related to PTH yet, unlike PTH, which is synthesized only by the parathyroid cells, PTH-rP is synthesized by several cell types. PTH-rP regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Isolated from the culture medium of a human lung cancer cell line, PTH-rP produces PTH-like effects that are characterized as humoral hypercalcemia of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol PTH