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PSMD7 Antibody

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Western Blot: PSMD7 Antibody [NBP2-19988] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:500.
Western Blot: PSMD7 Antibody [NBP2-19988] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: PSMD7 Antibody [NBP2-19988] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RM, Xp, ZeSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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PSMD7 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PSMD7. The exact sequence is proprietary.
Predicted Species
Mouse (98%), Rat (97%), Xenopus (96%), Zebrafish (90%), Rhesus Macaque (99%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSMD7
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for PSMD7 Antibody

  • 26S proteasome non-ATPase regulatory subunit 7
  • 26S proteasome regulatory subunit RPN8
  • Moloney leukemia virus-34 proviral integration
  • Mov34 homolog
  • Mov34 protein homolog
  • MOV3426S proteasome regulatory subunit S12
  • MOV34L
  • P40
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog)
  • proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
  • Proteasome subunit p40
  • Rpn8
  • S12

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PSMD7
Uniprot