![Western Blot: PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using CD162 antibody at a concentration of 2 ug/ml. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/PSGL-1-CD162-Antibody-HECA-452-Azide-and-BSA-Free-Western-Blot-NBP2-80921-img0001.jpg "Western Blot: PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - Analysis of human tonsil (1), human spleen (2), and human lymph node (3) tissue using CD162 antibody at a concentration of 2 ug/ml. Image from the standard format of this antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC, CyTOF-ready |
| Clone | HECA-452 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Human tonsil stroma |
| Specificity | CLA antibody clone HECA-452 is known to recognize the cutaneous lymphocyte-associated antigen / CLA which is a specialized glycosylated form of P-selectin glycoprotein ligand-1/PSGL-1. Besides lymphocytes, this clone has been shown to react with normal endometrium as well as breast cancer tissues (PMID 19625313 and 22970241). |
| Isotype | IgM Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | SELPLG |
| Purity | IgM purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | IgM purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for PSGL-1/CD162 Antibody (NBP2-80921)Find related products by research area.
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| Gene Symbol | SELPLG |
![Immunohistochemistry: PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast adenocarcinoma using CLA antibody (clone HECA-452) at 5 ug/ml concentration. The antibody generated a membrane-cytoplasmic staining in the cancerous cells of breast adenocarci](http://images.novusbio.com/fullsize/PSGL-1-CD162-Antibody-HECA-452-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80921-img0002.jpg "Immunohistochemistry: PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast adenocarcinoma using CLA antibody (clone HECA-452) at 5 ug/ml concentration. The antibody generated a membrane-cytoplasmic staining in the cancerous cells of breast adenocarci")
![Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - A cell surface stain was performed on CD3+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems]. A co-stain was performed using CD3 antibody NBP2-25186APC. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/PSGL-1-CD162-Antibody-HECA-452-Azide-and-BSA-Free-Flow-Cell-Surface-NBP2-80921-img0004.jpg "Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - A cell surface stain was performed on CD3+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems]. A co-stain was performed using CD3 antibody NBP2-25186APC. Image from the standard format of this antibody.")
![Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - A cell surface stain was performed on CD14+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by rat-Dylight 488 conjugated secondary antibody. A co-stain was performed using CD14 antibody NB100-77758AF488. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/PSGL-1-CD162-Antibody-HECA-452-Azide-and-BSA-Free-Flow-Cell-Surface-NBP2-80921-img0003.jpg "Flow (Cell Surface): PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - A cell surface stain was performed on CD14+ hPBMCs with Cutaneous Lymphocyte Antigen (CLA) antibody (HECA-452) NB100-78039 and a matched isotype control NBP2-31379. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by rat-Dylight 488 conjugated secondary antibody. A co-stain was performed using CD14 antibody NB100-77758AF488. Image from the standard format of this antibody.")
![Flow Cytometry: PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - Human peripheral blood lymphocytes stained with purified HECA-452, followed by anti-mouse IgG FITC Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/PSGL-1-CD162-Antibody-HECA-452-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80921-img0005.jpg "Flow Cytometry: PSGL-1/CD162 Antibody (HECA-452) - Azide and BSA Free [NBP2-80921] - Human peripheral blood lymphocytes stained with purified HECA-452, followed by anti-mouse IgG FITC Image from the standard format of this antibody.")
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