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Protein Kinase D2 Antibody (OTI5G1) - Azide and BSA Free

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Western Blot: Protein Kinase D2 Antibody (OTI5G1) - Azide and BSA Free [NBP2-73664] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase D2 (Right lane) cDNA for 48 hrs ...read more
Immunocytochemistry/ Immunofluorescence: Protein Kinase D2 Antibody (OTI5G1) - Azide and BSA Free [NBP2-73664] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein Kinase D2.
Immunohistochemistry: Protein Kinase D2 Antibody (OTI5G1) - Azide and BSA Free [NBP2-73664] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Protein Kinase D2 mouse monoclonal antibody.
Flow Cytometry: Protein Kinase D2 Antibody (OTI5G1) - Azide and BSA Free [NBP2-73664] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by ...read more
Western Blot: Protein Kinase D2 Antibody (OTI5G1) - Azide and BSA Free [NBP2-73664] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Protein Kinase D2 monoclonal antibody.
Immunohistochemistry: Protein Kinase D2 Antibody (OTI5G1) - Azide and BSA Free [NBP2-73664] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Protein Kinase D2 mouse monoclonal ...read more
Immunohistochemistry: Protein Kinase D2 Antibody (OTI5G1) - Azide and BSA Free [NBP2-73664] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Protein Kinase D2 mouse monoclonal antibody.
Immunohistochemistry: Protein Kinase D2 Antibody (OTI5G1) - Azide and BSA Free [NBP2-73664] - Staining of paraffin-embedded Human Kidney tissue using anti-Protein Kinase D2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI5G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Protein Kinase D2 Antibody (OTI5G1) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human PRKD2 (NP_057541) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PRKD2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
96.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Protein Kinase D2 Antibody (OTI5G1) - Azide and BSA Free

  • DKFZP586E0820
  • EC 2.7.11
  • EC 2.7.11.13
  • nPKC-D2
  • PKD2HSPC187
  • PRKD2
  • protein kinase D2
  • serine/threonine-protein kinase D2

Background

Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells; PKD2 is a novel phorbol ester and growth factor-stimulated protein kinase (1). PKD2 is widely expressed in human and murine tissues and in cell lines such as HL60. In vivo, phorbol ester has been shown to autophosphorylate PKD2 in a synergistic fashion. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu and PKC-nu (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKD2