Protein Kinase D2 Antibodies
We offer Protein Kinase D2 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Protein Kinase D2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Protein Kinase D2 Antibodies can be used in a variety of model species: Canine, Equine, Human, Mouse, Opossum, Primate, Rat. Use the list below to choose the Protein Kinase D2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Protein Kinase D2 polyclonal antibodies and browse our Protein Kinase D2 monoclonal antibody catalog.
Alternate Names for Protein Kinase D2 Antibodies
anti-Protein Kinase D2 antibody, anti-PRKD2 antibody, anti-DKFZP586E0820 antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.13 antibody, anti-nPKC-D2 antibody, anti-PKD2HSPC187 antibody, anti-serine/threonine-protein kinase D2 antibody
8 Results for "protein-kinase-d2" in Primary Antibodies
![Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase D2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protein Kinase D2.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Western-Blot-NBP2-02984-img0015.jpg "Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Protein Kinase D2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Protein Kinase D2.")
![Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Protein Kinase D2.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunocytochemistry-Immunofluorescence-NBP2-02984-img0010.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Human Kidney tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0014.jpg)
![Flow Cytometry: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Protein Kinase D2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Flow-Cytometry-NBP2-02984-img0009.jpg)
![Western Blot: Protein Kinase D2 Antibody (5G1) [NBP2-02984] Analysis of extracts (35ug) from 9 different cell lines by using anti-Protein Kinase D2 monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Western-Blot-NBP2-02984-img0008.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0011.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0012.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (5G1) [NBP2-02984] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Protein Kinase D2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-5G1-Immunohistochemistry-Paraffin-NBP2-02984-img0013.jpg)
![Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody NB100-636 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NB100-636-img0017.jpg "<b>Biological Strategies Validation. </b>Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody NB100-636 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PKD2 antibody (lot For blotting immunoprecipitated PKD2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NB100-636-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (A. 30 ug/lane; B. 200 mcg/IP) from HEK293 cells that were untreated or transfected with expression constructs for PKD1 and PKD3 as controls for reactivity with these homologs. Antibodies: A. Affinity purified rabbit anti- NB100-636 used at the indicated oncentrations for WB. B. Affinity purified rabbit anti- NB100-636 and NB100-2299 used at 2 ug/200 mcg lysate for IP. Immunoprecipitated PKD2 was detected by WB using anti-PKD2 antibody NB100-2299 at 1 ug/ml. Detection: Chemiluminescence with a 10 second exposure.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunoprecipitation-NB100-636-img0003.jpg)
![Western Blot: Protein Kinase D2 Antibody [NBP1-84139] - Analysis using Anti-PRKD2 antibody NBP1-84139 (A) shows similar pattern to independent antibody NBP2-58315 (B).](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NBP1-84139-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Protein Kinase D2 Antibody [NBP1-84139] - Analysis using Anti-PRKD2 antibody NBP1-84139 (A) shows similar pattern to independent antibody NBP2-58315 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining in human appendix and cerebral cortex tissues using anti-PRKD2 antibody. Corresponding PRKD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunohistochemistry-Paraffin-NBP1-84139-img0009.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining of human tonsil shows strong positivity in germinal and non-germinal center cells.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunohistochemistry-Paraffin-NBP1-84139-img0006.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunohistochemistry-Paraffin-NBP1-84139-img0007.jpg)
![Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunohistochemistry-Paraffin-NBP1-84139-img0008.jpg)
![Western Blot: Protein Kinase D2 Antibody [NBP2-99858] - Anti-Protein Kinase D2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 113 kDa](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NBP2-99858-img0004.jpg "Western Blot: Protein Kinase D2 Antibody [NBP2-99858] - Anti-Protein Kinase D2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 97 kDa. Observed band size: 113 kDa")
![Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody [NBP2-99858] - Immunofluorescence staining of Protein Kinase D2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Protein Kinase D2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99858-img0002.jpg)
![Immunoprecipitation: Protein Kinase D2 Antibody [NBP2-99858] - Protein Kinase D2 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate Lane B: 0.5 mg HepG2 Whole Cell Lysate Lane C: 0.5 mg MCF-7 Whole Cell Lysate 4 ul anti-Protein Kinase D2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Protein Kinase D2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 100 kDa](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunoprecipitation-NBP2-99858-img0001.jpg)
![Western Blot: Protein Kinase D2 Antibody [NBP2-58315] - Analysis using Anti-PRKD2 antibody NBP2-58315 (A) shows similar pattern to independent antibody NBP1-84139 (B).](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NBP2-58315-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Protein Kinase D2 Antibody [NBP2-58315] - Analysis using Anti-PRKD2 antibody NBP2-58315 (A) shows similar pattern to independent antibody NBP1-84139 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Protein Kinase D2 Antibody [NBP2-58315] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58315-img0001.jpg)