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Protein Kinase D2 Antibody

Images

 
Independent Antibodies: Western Blot: Protein Kinase D2 Antibody [NBP1-84139] - Analysis using Anti-PRKD2 antibody NBP1-84139 (A) shows similar pattern to independent antibody NBP2-58315 (B).
Orthogonal Strategies: Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining in human appendix and cerebral cortex tissues using anti-PRKD2 antibody. Corresponding PRKD2 RNA-seq data ...read more
Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining of human tonsil shows strong positivity in germinal and non-germinal center cells.
Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining of human appendix shows high expression.
Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody [NBP1-84139] - Staining of human cerebral cortex shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Protein Kinase D2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSGLPTDRDLGGACPPQDHDMQGLAERISVL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRKD2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Protein Kinase D2 Protein (NBP1-84139PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Protein Kinase D2 Antibody

  • DKFZP586E0820
  • EC 2.7.11
  • EC 2.7.11.13
  • nPKC-D2
  • PKD2HSPC187
  • PRKD2
  • protein kinase D2
  • serine/threonine-protein kinase D2

Background

Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells; PKD2 is a novel phorbol ester and growth factor-stimulated protein kinase (1). PKD2 is widely expressed in human and murine tissues and in cell lines such as HL60. In vivo, phorbol ester has been shown to autophosphorylate PKD2 in a synergistic fashion. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu and PKC-nu (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PRKD2