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Proteasome 20S beta 6 Antibody (S51)

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Western Blot: Proteasome 20S beta 6 Antibody (S51) [H00005694-M02] - PSMB6 monoclonal antibody (M02), clone S51 Analysis of PSMB6 expression in A-431.
Western Blot: Proteasome 20S beta 6 Antibody (S51) [H00005694-M02] - PSMB6 monoclonal antibody (M02), clone S51 Analysis of PSMB6 expression in A-431.
Immunocytochemistry/ Immunofluorescence: Proteasome 20S beta 6 Antibody (S51) [H00005694-M02] - Analysis of monoclonal antibody to PSMB6 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Proteasome 20S beta 6 Antibody (S51) [H00005694-M02] - Analysis of monoclonal antibody to PSMB6 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.
ELISA: Proteasome 20S beta 6 Antibody (S51) [H00005694-M02] - Detection limit for recombinant GST tagged PSMB6 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
S51
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Proteasome 20S beta 6 Antibody (S51) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PSMB6 (AAH00835, 1 a.a. ~ 239 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAATLLAARGAGPAPAWGPEAFTPDWESREVSTGTTIMAVQFDGGVVLGADSRTTTGSYIANRVTDELTPIHDRIFCCRSGSAADTQAVADAVTYQLGFHSIELNEPPLVHTAASLFKEMCYRYREDLMAGIIIAGWDPQEGGQVYSVPMGGMMVRQSFAIGGSGSSYIYGYVDATYREGMTKEECLQFTANALALAMERDGSSGGVIRLAAIAESGVERQVLLGDQIPKFAVATLPPA
Specificity
PSMB6 - proteasome (prosome, macropain) subunit, beta type, 6
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PSMB6
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Proteasome 20S beta 6 Antibody (S51)

  • DELTA
  • EC 3.4.25.1
  • LMPY
  • Macropain delta chain
  • Multicatalytic endopeptidase complex delta chain
  • proteasome (prosome, macropain) subunit, beta type, 6
  • Proteasome 20S beta 6
  • proteasome catalytic subunit 1
  • Proteasome delta chain
  • proteasome subunit beta 6
  • proteasome subunit beta type-6
  • proteasome subunit delta
  • Proteasome subunit Y
  • PSMB6
  • PSY large multifunctional protease Y
  • YMGC5169

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 1i (proteasome beta 9 subunit).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PSMB6
Entrez
OMIM
Uniprot