Home » Pref-1/DLK1/FA1 » Pref-1/DLK1/FA1 Antibodies » Pref-1/DLK1/FA1 Antibody (211309) [Alexa Fluor® 488]
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Pref-1/DLK1/FA1 Antibody (211309) [Alexa Fluor® 488]

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HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1144G, filled histogram) or isotype control antibody (IC0041G, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Pref-1/DLK1/FA1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1144G, filled histogram) or isotype control antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
211309
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1144U-100UG
Alexa Fluor 405
FAB1144V-100UG
Alexa Fluor 488
FAB1144G-025
Alexa Fluor 532
FAB1144X
Alexa Fluor 594
FAB1144T-100UG
Alexa Fluor 647
FAB1144R-100UG
Alexa Fluor 700
FAB1144N-100UG
Alexa Fluor 750
FAB1144S-100UG
Allophycocyanin
FAB1144A-100
Allophycocyanin
FAB1144A-025
Allophycocyanin/Cy7
FAB1144APCCY7
Biotin
FAB1144B
CoraFluor 1
FAB1144CL1
DyLight 350
FAB1144D
DyLight 405
FAB1144E
DyLight 488
FAB1144K
DyLight 550
FAB1144L
DyLight 594
FAB1144M
DyLight 650
FAB1144W
DyLight 680
FAB1144Y
DyLight 755
FAB1144Z
FITC
FAB1144F
HRP
FAB1144H
Janelia Fluor 525
FAB1144JF525
Janelia Fluor 549
FAB1144I
Janelia Fluor 646
FAB1144J
Janelia Fluor 669
FAB1144JF669
PE
FAB1144P
PE/Atto594
FAB1144PEATT594
PE/Cy5.5
FAB1144PECY55
PE/Cy7
FAB1144PECY7
PerCP
FAB1144C
Unconjugated
MAB1144-100
mFluor Violet 450 SE
FAB1144MFV450
mFluor Violet 500 SE
FAB1144MFV500
mFluor Violet 610 SE
FAB1144MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1144-100
Carrier Free
MAB1144-SP
Carrier Free
FAB1144G-025
Carrier Free
FAB1144A-100
Carrier Free
FAB1144A-025
Carrier Free
MAB1144-500
Carrier Free
MAB1144-100
Carrier Free
FAB1144V-100UG
Carrier Free
FAB1144U-100UG
Carrier Free
FAB1144T-100UG
Carrier Free
FAB1144S-100UG
Carrier Free
FAB1144R-100UG
Carrier Free
FAB1144N-100UG
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Pref-1/DLK1/FA1 Antibody (211309) [Alexa Fluor® 488] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pref-1/DLK1/FA1 long isoform
Ala24-Pro297 (with Arg248Pro and Lys295Ser substitutions)
Accession # P80370
Specificity
Detects human Pref‑1/DLK1/FA1 in direct ELISAs and Western blots. Shows approximately 50% cross-reactivity with recombinant mouse Pref‑1/DLK1/FA1.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DLK1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 5 uL/10^6 cells
Publications
Read Publications using
FAB1144G in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pref-1/DLK1/FA1 Antibody (211309) [Alexa Fluor® 488]

  • delta-like 1 homolog (Drosophila)
  • DLK
  • DLK1
  • DLK-1
  • FA1
  • FA1fetal antigen 1
  • pG2
  • pG2delta-like homolog (Drosophila)
  • preadipocyte factor 1
  • Pref1
  • Pref-1
  • protein delta homolog 1
  • secredeltin
  • ZOG

Background

Pref-1 (Preadipocyte Factor 1), also known as DLK1 and FA1, is a 58-65 kDa member of the Notch/Serrata/Delta family of proteins.  It is expressed in prechondrocytes and preadipocytes and appears to block progenitor cell differentiation into mature cell lineages.  Mature human Pref-1 is a 360 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 280 aa extracellular region (aa 24-303), a 24 aa transmembrane segment (aa 304-327), and a 56 aa cytoplasmic domain (aa 328-383).  The extracellular region contains six EGF-like domains, and undergoes proteolytic cleavage to generate a bioactive 50 kDa fragment, plus three 25-31 kDa fragments that show no activity.  There are multiple potential splice variants.  One shows a deletion of aa 229-301, a second possesses a six aa substitution for aa 1-52, a third shows a deletion of aa 210-277, while a fourth contains a six aa substitution for aa 207-383.  Over aa 24-297, human Pref-1 shares 82% aa identity with mouse Pref-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Pref-1/DLK1/FA1
Antibody (211309) [Al ...
FAB1144G
Pref-1/DLK1/FA1 Antibody (211309) [Alexa Fluor® 488]
Species: Hu
Applications: Flow

Publications for Pref-1/DLK1/FA1 Antibody (FAB1144G)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FACS, Flow Cytometry.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DLK1
Uniprot