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PP2A alpha Antibody

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Western Blot: PP2A alpha Antibody [NB100-844] - Staining of Mouse (A), Rat (B) and Pig (C) Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Western Blot: PP2A alpha Antibody [NB100-844] - Staining of Mouse (A), Rat (B) and Pig (C) Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PP2A alpha Antibody [NB100-844] - )Staining of paraffin embedded Human Thyroid. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: PP2A alpha Antibody [NB100-844] - Staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PP2A alpha Antibody [NB100-844] - Staining of paraffin embedded Human Kidney. Antibody at 10 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, RbSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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PP2A alpha Antibody Summary

Immunogen
Peptide with sequence C-EPHVTRRTPDYFL corresponding to C-Terminus according to NP_004147.1.
Specificity
Two variants NP_001009552.1 and NP_004147.1 represent identical protein.
Predicted Species
Canine (100%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PPP2CA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/mL
Application Notes
WB: Approx. 36 kDa band observed in human brain (cerebellum), mouse, rat and porcine brain lysates (calculated MW of 35.6 kDa band according to human NP_002706.1, mouse NP_062284.1, rat NP_058735.1 and porcine NP_999531.1).
Control
PP2A alpha Overexpression Lysate
Publications
Read Publication using NB100-844.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PP2A alpha Antibody

  • EC 3.1.3.16
  • PP2A
  • PP2A-alpha
  • PP2Ac
  • PP2CA
  • PP2Calpha
  • PPP2CA
  • protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
  • protein phosphatase 2, catalytic subunit, alpha isozyme
  • protein phosphatase 2A catalytic subunit, alpha isoform
  • Replication protein C
  • RP-C
  • serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
  • serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Background

Functions in cell cycle control and growth factor signaling. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. It can also dephosphorylate SV40 large T antigen and p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PP2A alpha Antibody (NB100-844)(1)


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Control Lysate(s)

PP2A alpha Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPP2CA
Entrez
Uniprot