![Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Mouse kidney tissue with Rabbit anti-BCL2A1 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Bcl-2-related-protein-A1-Antibody-SC05-42-Immunohistochemistry-Paraffin-NBP2-67563-img0007.jpg "Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Mouse kidney tissue with Rabbit anti-BCL2A1 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clone | SC05-42 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human Bcl-2 related protein A1 aa 2-48 / 175. (SwissProt: Q16548 Human; SwissProt: Q07440 Mouse; Unigene:19770 Rat) |
| Localization | Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | BCL2A1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot RFC1 Antibody [Unconjugated]](https://images.novusbio.com/images2/RFC1_AF6457_Western_Blot_9874.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Bcl-2 related protein A1 Antibody (NBP2-67563)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BCL2A1 |
![Flow Cytometry: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of 293 cells with Bfl1(BCL2A1) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Bcl-2-related-protein-A1-Antibody-SC05-42-Flow-Cytometry-NBP2-67563-img0001.jpg "Flow Cytometry: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of 293 cells with Bfl1(BCL2A1) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of paraffin-embedded human kidney tissue using anti- BCL2A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-related-protein-A1-Antibody-SC05-42-Immunohistochemistry-Paraffin-NBP2-67563-img0002.jpg "Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of paraffin-embedded human kidney tissue using anti- BCL2A1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of paraffin-embedded human tonsil tissue using anti-BCL2A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-related-protein-A1-Antibody-SC05-42-Immunohistochemistry-Paraffin-NBP2-67563-img0003.jpg "Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of paraffin-embedded human tonsil tissue using anti-BCL2A1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of paraffin-embedded mouse kidney tissue using anti- BCL2A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-related-protein-A1-Antibody-SC05-42-Immunohistochemistry-Paraffin-NBP2-67563-img0004.jpg "Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of paraffin-embedded mouse kidney tissue using anti- BCL2A1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of paraffin-embedded mouse liver tissue using anti- BCL2A1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bcl-2-related-protein-A1-Antibody-SC05-42-Immunohistochemistry-Paraffin-NBP2-67563-img0005.jpg "Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Analysis of paraffin-embedded mouse liver tissue using anti- BCL2A1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Human kidney tissue with Rabbit anti-BCL2A1 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Bcl-2-related-protein-A1-Antibody-SC05-42-Immunohistochemistry-Paraffin-NBP2-67563-img0006.jpg "Immunohistochemistry-Paraffin: Bcl-2 related protein A1 Antibody (SC05-42) [NBP2-67563] - Human kidney tissue with Rabbit anti-BCL2A1 antibody at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot PP2A alpha [p Tyr307] Antibody [Unconjugated]](https://images.novusbio.com/images2/PP2A_AF3989_Western_Blot_5373.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Western Blot c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Western_Blot_18923.jpg)
![Immunocytochemistry c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Immunocytochemistry__Immunofluorescence_18945.jpg)
![Simple Western c-jun [p Ser63] Antibody (1018Y) [Unconjugated]](https://images.novusbio.com/images2/cJun_MAB8930_Simple_Western_19175.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")