Home » PON3 » PON3 Antibodies » PON3 Antibody [Unconjugated]

PON3 Antibody [Unconjugated]

 (2 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western blot shows human serum, lysates of A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line, mouse liver and spleen tissue, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat PON3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4345) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PON3 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows human serum, lysates of A549 human lung carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line, mouse liver and spleen tissue, and rat liver tissue. PVDF membrane was probed with 0.5 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4345-SP
Novus Biologicals offers 100% Guarantee

PON3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PON3
Ala30-Leu354
Accession # Q15166
Specificity
Detects endogenous human, mouse, and rat PON3 in Western blots. In Western blots, this antibody did not cross‑react with recombinant human PON1 or PON2.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PON3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF4345 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PON3 Antibody [Unconjugated]

  • EC 3.1.1.2
  • EC 3.1.1.81
  • EC 3.1.8.1
  • paraoxanase-3
  • paraoxonase 3
  • PON3
  • serum paraoxonase/lactonase 3

Background

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/lactonase 3 (PON3) is a 354 amino acid, 39.6 kDa protein that is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 has lactonase activity (EC 3.1.1) and rapidly hydrolyzes lactones. PON3 exhibits no paraoxonase and very limited arylesterase activities. PON3 is an important antioxidant and can inhibit the oxidation of low-density lipoprotein (LDL), a function that may slow the initiation and progression of atherosclerosis. PON3 is glycosylated at Asn323. Alternatively spliced variants encoding different protein isoforms have been described; however, only one has been fully characterized. Human PON3 shares 81% amino acid identity with mouse and rat PON3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

PON1 Antibody
(4G8D3) - Azide and B ...
NBP2-80919
Western Blot PON1 Antibody (4G8D3) - Azide and BSA Free
Western Blot PON1 Antibody (4G8D3) - Azide and BSA Free
Western Blot PON1 Antibody (4G8D3) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF (-), IHC, IHC-P, WB
PON2 Antibody
AF4344
Western Blot PON2 Antibody
(2 Publications)
Species: Hu
Applications: WB
SOD1/Cu-Zn SOD
Antibody - BSA Free
NBP2-24915
Western Blot SOD1/Cu-Zn SOD Antibody - BSA Free
Western Blot SOD1/Cu-Zn SOD Antibody - BSA Free
Immunohistochemistry-Paraffin SOD1/Cu-Zn SOD Antibody - BSA Free
(3 Reviews)
(21 Publications)
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
CCL2/JE/MCP-1 [HRP]
DCP00
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
N/A CCL2/JE/MCP-1 [HRP]
(258 Publications)
Species: Hu
Applications: ELISA
ALS Antibody
[Unconjugated]
AF1436
Western Blot ALS Antibody [Unconjugated]
(1 Review)
(3 Publications)
Species: Mu
Applications: WB
Apolipoprotein
A-I/ApoA1 Antibody - ...
NBP2-52979
Flow Cytometry Apolipoprotein A-I/ApoA1 Antibody - BSA Free
Western Blot Apolipoprotein A-I/ApoA1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Apolipoprotein A-I/ApoA1 Antibody - BSA Free
(1 Review)
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(822 Publications)
Species: Hu
Applications: BA
LDLR Antibody
[Unconjugated]
AF2255
Immunohistochemistry LDLR Antibody [Unconjugated]
Western Blot LDLR Antibody [Unconjugated]
Western Blot LDLR Antibody [Unconjugated]
(47 Publications)
Species: Mu
Applications: Block, CyTOF-ready, Flow, IHC, WB
Thyroglobulin
Antibody (2H11 + 6E1)
NBP2-34294
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry Thyroglobulin Antibody (2H11 + 6E1)
Immunohistochemistry-Paraffin Thyroglobulin Antibody (2H11 + 6E1)
(3 Reviews)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
Serpin
A8/Angiotensinogen An ...
NBP1-30027
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
Western Blot Serpin A8/Angiotensinogen Antibody - BSA Free
Immunohistochemistry-Paraffin Serpin A8/Angiotensinogen Antibody - BSA Free
(12 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
C-Reactive
Protein/CRP [Biotin]
DY1707
N/A C-Reactive Protein/CRP [Biotin]
(71 Publications)
Species: Hu
Applications: ELISA
Myocilin Antibody
(297817) [Unconjugate ...
MAB3446
Immunohistochemistry Myocilin Antibody (297817) [Unconjugated]
Western Blot Myocilin Antibody (297817) [Unconjugated]
Western Blot Myocilin Antibody (297817) [Unconjugated]
(1 Review)
(14 Publications)
Species: Hu
Applications: IHC
Apolipoprotein
E/ApoE Antibody (WUE- ...
NB110-60531
Flow (Intracellular) Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
Western Blot Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
Flow Cytometry Apolipoprotein E/ApoE Antibody (WUE-4) - BSA Free
(2 Reviews)
(41 Publications)
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
TTF2 Antibody
(1E8) - Azide and BSA ...
H00008458-M06
Immunocytochemistry/ Immunofluorescence TTF2 Antibody (1E8) - Azide and BSA Free
Western Blot TTF2 Antibody (1E8) - Azide and BSA Free
Western Blot TTF2 Antibody (1E8) - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
Cytochrome P450
2A6 Antibody (OTI1D2)
NBP2-01437
Western Blot Cytochrome P450 2A6 Antibody (OTI1D2)
Immunohistochemistry-Paraffin Cytochrome P450 2A6 Antibody (OTI1D2)
Western Blot Cytochrome P450 2A6 Antibody (OTI1D2)
Species: Hu, Pm, Mu, Pm, Rt
Applications: Flow, IHC, IHC-P, WB
PON3 Antibody
[Unconjugated]
AF4345
PON3 Antibody [Unconjugated]
Species: Hu, Mu, Rt
Applications: WB

Publications for PON3 Antibody (AF4345)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(2)
All Applications
Filter By Species
Human
(2)
All Species

Reviews for PON3 Antibody (AF4345) (0)

There are no reviews for PON3 Antibody (AF4345). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PON3 Antibody (AF4345) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional PON3 Products

Blogs on PON3

There are no specific blogs for PON3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog #F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PON3 Antibody [Unconjugated] and receive a gift card or discount.


Bioinformatics

Gene Symbol PON3
Uniprot