Myocilin Antibody (297817) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Myocilin (also known as TIGR) is a variably glycosylated, 65 kDa, secreted polypeptide that belongs to the family of olfactomedin-related proteins. Human Myocilin is synthesized as a 490 aa precursor that contains an 18 aa signal sequence, an 84 aa N-terminus, a 53 aa alpha ‑helical leucine zipper and a 335 aa C-terminus that contains a 260 aa OLF-domain. An alternate start site generates a signal-sequenceless 504 aa mature protein. Myocilin forms nondisulfide-linked dimers and multimers. The human 32 kDa OLF-domain shares 87% aa sequence identity with the OLF-domain in bovine, rat, and mouse.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Tree Shrew.

We have publications tested in 3 applications: IHC, Immunoprecipitation, Western Blot.

Showing Publications 1 - 10 of 14. Show All 14 Publications. Collapse Publications.

Publications using MAB3446	Applications	Species
HF Scelsi, KR Hill, BM Barlow, MD Martin, RL Lieberman Disambiguation of benign and misfolded glaucoma-causing myocilin variants on the basis of protein thermal stability Disease Models & Mechanisms, 2023-01-13;0(0):. 2023-01-13 [PMID: 36579626] (Western Blot, Human)	Western Blot	Human
Emily G. Saccuzzo, Mackenzie D. Martin, Kamisha R. Hill, Minh Thu Ma, Yemo Ku, Raquel L. Lieberman Calcium dysregulation potentiates wild-type myocilin misfolding: implications for glaucoma pathogenesis JBIC Journal of Biological Inorganic Chemistry 2022-07-13 [PMID: 35831671]
JV Jasien, AT Read, J van Batenb, KM Perkumas, CR Ethier, WD Stamer, BC Samuels Anterior Segment Anatomy and Conventional Outflow Physiology of the Tree Shrew (Tupaia belangeri) Investigative Ophthalmology & Visual Science, 2022-01-03;63(1):21. 2022-01-03 [PMID: 35040876] (IHC, Tree Shrew)	IHC	Tree Shrew
Mackenzie D. Martin, Dustin J. E. Huard, Ricardo C. Guerrero-Ferreira, Ishani M. Desai, Brett M. Barlow, Raquel L. Lieberman Molecular architecture and modifications of full-length myocilin Experimental Eye Research 2021-10-01 [PMID: 34400147]
AC Patterson-, AN Qerqez, LR Azouz, MT Ma, SE Hill, Y Ku, LA Schildmeye, JA Maynard, RL Lieberman Recombinant antibodies recognize conformation-dependent epitopes of the leucine zipper of misfolding-prone myocilin The Journal of Biological Chemistry, 2021-08-09;0(0):101067. 2021-08-09 [PMID: 34384785] (Western Blot, Human)	Western Blot	Human
Siqi Xiong, Ajay Kumar, Shenghe Tian, Eman E Taher, Enzhi Yang, Paul R Kinchington et al. Stem cell transplantation rescued a primary open-angle glaucoma mouse model eLife 2021-01-28 [PMID: 33506763]
R Haribalaga, C Gowri Priy, R Sharmila, S Krishnadas, V Muthukkaru, CE Willoughby, S Senthilkum Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes Scientific Reports, 2021-01-12;11(1):605. 2021-01-12 [PMID: 33436790] (IHC, Human)	IHC	Human
Julia Staverosky, Kamesh Dhamodaran, Ted Acott, VijayKrishna Raghunathan, Janice Vranka Isolation and Characterization of primary human trabecular meshwork cells from segmental flow regions: New tools for understanding segmental flow Experimental Eye Research 2020-08-01 [PMID: 32376472]
SE Hill, MS Kwon, MD Martin, A Suntharali, A Hazel, CA Dickey, JC Gumbart, RL Lieberman Stable calcium-free myocilin olfactomedin domain variants reveal challenges in differentiating between�benign and glaucoma-causing mutations J. Biol. Chem., 2019-07-02;0(0):. 2019-07-02 [PMID: 31270212] (Western Blot, Human)	Western Blot	Human
José-Daniel Aroca-Aguilar, Ana Fernández-Navarro, Jesús Ontañón, Miguel Coca-Prados, Julio Escribano Identification of myocilin as a blood plasma protein and analysis of its role in leukocyte adhesion to endothelial cell monolayers PLOS ONE 2018-12-17 [PMID: 30557320]
Athéna C. Patterson-Orazem, Shannon E. Hill, Michael P. Fautsch, Raquel L. Lieberman Epitope mapping of commercial antibodies that detect myocilin Experimental Eye Research 2018-08-01 [PMID: 29752947]
Kate E. Keller, Sanjoy K. Bhattacharya, Theresa Borrás, Thomas M. Brunner, Sunee Chansangpetch, Abbott F. Clark et al. Consensus recommendations for trabecular meshwork cell isolation, characterization and culture Experimental Eye Research 2018-06-01 [PMID: 29526795]
Suntharalingam A, Abisambra J, O'Leary J, Koren J, Zhang B, Joe M, Blair L, Hill S, Jinwal U, Cockman M, Duerfeldt A, Tomarev S, Blagg B, Lieberman R, Dickey C Glucose-regulated protein 94 triage of mutant myocilin through endoplasmic reticulum-associated degradation subverts a more efficient autophagic clearance mechanism. J Biol Chem, 2012-10-03;287(48):40661-9. 2012-10-03 [PMID: 23035116] (Immunoprecipitation, Human)	Immunoprecipitation	Human
Joe MK, Tomarev SI Expression of myocilin mutants sensitizes cells to oxidative stress-induced apoptosis: implication for glaucoma pathogenesis. Am. J. Pathol., 2010-04-09;176(6):2880-90. 2010-04-09 [PMID: 20382707] (Western Blot, Human)	Western Blot	Human
Show All 14 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	ICC	Pig	01/04/2017	View Summary Application Immunocytochemistry Sample Tested Pig TM cells Species Pig Comments Comments IHC showing detection of Myocilin using anti-Myocilin antibody (MAB3446) at 1:1000 dilution in Pig TM cells.

Array MAB3446

• Myocilin Antibodies
• Myocilin ELISA Kits
• Myocilin Lysate
• Myocilin Proteins
• Myocilin Proteins and Enzyme