![Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Western blot analysis of PLGF on JAR cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Western-Blot-NBP2-67067-img0009.jpg "Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Western blot analysis of PLGF on JAR cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | JA63-15 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human PLGF aa 48-97 / 221. (SwissProt: P49763 Human; SwissProt: P49764 Mouse; SwissProt: Q63434 Rat) |
| Localization | Secreted. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PGF |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Immunocytochemistry Endoglin/CD105 Antibody [Unconjugated]](https://images.novusbio.com/images2/Endoglin_AF1320_Immunocytochemistry_7258.jpg)
![Immunocytochemistry Endoglin/CD105 Antibody [Unconjugated]](https://images.novusbio.com/images2/Endoglin_AF1320_Immunocytochemistry_12779.jpg)
![Simple Western Endoglin/CD105 Antibody [Unconjugated]](https://images.novusbio.com/images2/Endoglin_AF1320_Simple_Western_18976.jpg)
![Neutralization VEGFR2/KDR/Flk-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/VEGF_R2_AF644_Block_Neutralize_1519.jpg)
![Immunohistochemistry VEGFR2/KDR/Flk-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/VEGF_R2_AF644_Immunohistochemistry_22159.jpg)
![Immunohistochemistry VEGFR2/KDR/Flk-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/VEGF_R2_AF644_Immunohistochemistry_6888.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for PLGF Antibody (NBP2-67067)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PGF |
![Immunocytochemistry/Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067] - Staining PLGF in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunocytochemistry-Immunofluorescence-NBP2-67067-img0002.jpg "Immunocytochemistry/Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067] - Staining PLGF in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded rat kidney tissue using anti-PLGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunohistochemistry-Paraffin-NBP2-67067-img0006.jpg "Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded rat kidney tissue using anti-PLGF antibody. Counter stained with hematoxylin.")
![Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of PLGF on SH-SY5Y cell lysate using anti-PLGF antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Western-Blot-NBP2-67067-img0007.jpg "Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of PLGF on SH-SY5Y cell lysate using anti-PLGF antibody at 1/1,000 dilution.")
![Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of PLGF on JAR cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Western-Blot-NBP2-67067-img0008.jpg "Western Blot: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of PLGF on JAR cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067] - Staining PLGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunocytochemistry-Immunofluorescence-NBP2-67067-img0001.jpg "Immunocytochemistry/Immunofluorescence: PLGF Antibody (JA63-15) [NBP2-67067] - Staining PLGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded human colon cancer tissue using anti-PLGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunohistochemistry-Paraffin-NBP2-67067-img0003.jpg "Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded human colon cancer tissue using anti-PLGF antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded human liver cancer tissue using anti-PLGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunohistochemistry-Paraffin-NBP2-67067-img0004.jpg "Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded human liver cancer tissue using anti-PLGF antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded mouse orchis tissue using anti-PLGF antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PLGF-Antibody-JA63-15-Immunohistochemistry-Paraffin-NBP2-67067-img0005.jpg "Immunohistochemistry-Paraffin: PLGF Antibody (JA63-15) [NBP2-67067] - Analysis of paraffin-embedded mouse orchis tissue using anti-PLGF antibody. Counter stained with hematoxylin.")
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Immunohistochemistry VEGFR1/Flt-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/VEGF_R1_AF321_Immunohistochemistry_6778.jpg)
![Immunohistochemistry VEGFR1/Flt-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/VEGF_R1_AF321_Immunohistochemistry_9337.jpg)
![Western Blot VEGFR1/Flt-1 Antibody [Unconjugated]](https://images.novusbio.com/images/af321_human-vegf-r1-flt-1-affinity-purified-polyclonal-ab-8220249511449.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Bioactivity CTLA-4 [Unconjugated]](https://images.novusbio.com/images2/CTLA4_7268CT_2293.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")