![Western Blot: PIP Antibody (PIP/1571) [NBP2-53226] - Western Blot Analysis (A) Human Pancreas (B) HepG2 Cell lysate Using GCDFP-15 Monoclonal Antibody (PIP/1571).](http://images.novusbio.com/fullsize/PIP-Antibody-PIP-1571-Western-Blot-NBP2-53226-img0002.jpg "Western Blot: PIP Antibody (PIP/1571) [NBP2-53226] - Western Blot Analysis (A) Human Pancreas (B) HepG2 Cell lysate Using GCDFP-15 Monoclonal Antibody (PIP/1571).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, MA |
| Clone | PIP/1571 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54451) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human PIP protein fragment (around aa 41-146) (exact sequence is proprietary) (Uniprot: P12273) |
| Localization | Cytoplasmic (Golgi bodies) and Secreted |
| Marker | Breast Marker |
| Specificity | It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PIP |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PIP Antibody (NBP2-53226)Find related products by research area.
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![Immunohistochemistry-Paraffin: PIP Antibody (PIP/1571) [NBP2-53226] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GCDFP-15 Monoclonal Antibody (PIP/1571).](http://images.novusbio.com/fullsize/PIP-Antibody-PIP-1571-Immunohistochemistry-Paraffin-NBP2-53226-img0001.jpg "Immunohistochemistry-Paraffin: PIP Antibody (PIP/1571) [NBP2-53226] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GCDFP-15 Monoclonal Antibody (PIP/1571).")
![Protein Array: PIP Antibody (PIP/1571) [NBP2-53226] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PIP Antibody (PIP/1571). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/PIP-Antibody-PIP-1571-Protein-Array-NBP2-53226-img0004.jpg "Protein Array: PIP Antibody (PIP/1571) [NBP2-53226] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PIP Antibody (PIP/1571). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
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