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Phosphodiesterase 4A/PDE4A Antibody

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Western Blot: Phosphodiesterase 4A/PDE4A Antibody [H00005141-B01P] - Analysis of PDE4A expression in transfected 293T cell line by PDE4A polyclonal antibody. Lane 1: PDE4A transfected lysate(71.17 KDa). Lane 2: Non-transfected lysate.
Western Blot: Phosphodiesterase 4A/PDE4A Antibody [H00005141-B01P] - Analysis of PDE4A expression in transfected 293T cell line by PDE4A polyclonal antibody. Lane 1: PDE4A transfected lysate(71.17 KDa). Lane 2: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

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Phosphodiesterase 4A/PDE4A Antibody Summary

Description
Quality control test: Antibody reactive against mammalian transfected lysate.
Immunogen
PDE4A (NP_006193.1, 1 a.a. - 647 a.a.) full-length human protein. MPLVDFFCETCSKPWLVGWWDQFKRMLNRELTHLSEMSRSGNQVSEYISTTFLDKQNEVEIPSPTMKEREKQQAPRPRPSQPPPPPVPHLQPMSQITGLKKLMHSNSLNNSNIPRFGVKTDQEELLAQELENLNKWGLNIFCVSDYAGGRSLTCIMYMIFQERDLLKKFRIPVDTMVTYMLTLEDHYHADVAYHNSLHAADVLQSTHVLLATPALDAVFTDLEILAALFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEDNCDIFQNLSKRQRQSLRKMVIDMVLATDMSKHMTLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLRNMVHCADLSNPTKPLELYRQWTDRIMAEFFQQGDRERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVHPDAQEILDTLEDNRDWYYSAIRQSPSPPPEEESRGPGHPPLPDKFQFELTLEEEEEEEISMAQIPCTAQEALTAQGLSGVEEALDATIAWEASPAQESLEVMAQEASLEAELEAVYLTQQAQSTGSAPVAPDEFSSREEFVVAVSHSSPSALALQSPLLPAWRTLSVSEHAPGLPGLPSTAAEVEAQREHQAAKRACSACAGTFGEDTSALPAPGGGGSGGDPT
Specificity
PDE4A - phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila),
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
PDE4A
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Phosphodiesterase 4A/PDE4A Antibody

  • cAMP-specific 3'-5'-cyclic phosphodiesterase 4A
  • DPDE2
  • DPDE2cAMP-specific phosphodiesterase
  • EC 3.1.4
  • EC 3.1.4.17
  • PDE4
  • PDE46
  • PDE4A
  • Phosphodiesterase 4A
  • phosphodiesterase 4A, cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E2)
  • phosphodiesterase 4A, cAMP-specific (dunce
  • phosphodiesterase 4A, cAMP-specific
  • phosphodiesterase E2 dunce homolog, Drosophila
  • phosphodiesterase isozyme 4

Background

Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are also important molecular targets for a variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants. Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases. Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling. In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Phosphodiesterase
4A/PDE4A Antibody
H00005141-B01P
Phosphodiesterase 4A/PDE4A Antibody
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol PDE4A
Entrez
Uniprot