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Rat Periostin/OSF-2 ELISA Kit (Colorimetric)

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ELISA: Rat Periostin/OSF-2 ELISA Kit (Colorimetric) [NBP2-82144] - Samples were spiked with high concentrations of Rat Periostin/OSF-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Periostin/OSF-2 ELISA Kit (Colorimetric) [NBP2-82144] - Samples were spiked with high concentrations of Rat Periostin/OSF-2 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Rat Periostin/OSF-2 ELISA Kit (Colorimetric) [NBP2-82144] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
125 - 8000 pg/mL
Sensitivity
75 pg/mL

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Rat Periostin/OSF-2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat POSTN/OSF-2 in samples. No Significant cross-reactivity or interference between Rat POSTN/OSF-2 and analogues was observed.
Standard Curve Range
125 - 8000 pg/mL
Sensitivity
75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 6%
Intra-Assay
CV% < 6%
Spike Recovery
average = 92% for serum; average = 97% for EDTA plasma; average = 93% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
POSTN

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Rat Periostin/OSF-2 ELISA Kit (Colorimetric)

  • Fasciclin I-like
  • MGC119510
  • MGC119511
  • OSF2
  • OSF-2
  • OSF-2osteoblast specific factor 2 (fasciclin I-like)
  • OSF2periodontal ligament-specific periostin
  • Osteoblast-specific factor 2
  • PDLPOSTN
  • periostin isoform thy2
  • periostin isoform thy4
  • periostin isoform thy6
  • periostin isoform thy8
  • Periostin
  • periostin, osteoblast specific factor
  • PNRP11-412K4.1
  • POSTN
  • TRIF52

Background

Periostin is a disulfide linked 90 kDa 811 amno acid protein originally isolated as a osteoblastspecific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP)-2. OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Rat Periostin/OSF-2 ELISA Kit (Colorimetric) Manual
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Bioinformatics

Gene Symbol POSTN