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PEG10 Antibody (981902) [Unconjugated]

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HepG2 human hepatoma cell line was stained with Mouse Anti-Human PEG-10 Monoclonal Antibody (Catalog # MAB99751, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
HepG2 human hepatoma cell line was stained with Mouse Anti-Human PEG-10 Monoclonal Antibody (Catalog # MAB99751, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, CyTOF-ready, Flow
Clone
981902
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC99751U-100UG
Alexa Fluor 405
IC99751V-100UG
Alexa Fluor 488
IC99751G-100UG
Alexa Fluor 532
FAB99751X
Alexa Fluor 647
IC99751R-100UG
Alexa Fluor 700
IC99751N-100UG
Alexa Fluor 750
IC99751T-100UG
Alexa Fluor 750
IC99751S-100UG
Allophycocyanin
FAB99751A
Biotin
FAB99751B
DyLight 350
FAB99751D
DyLight 405
FAB99751E
DyLight 488
FAB99751K
DyLight 550
FAB99751L
DyLight 594
FAB99751M
DyLight 650
FAB99751W
DyLight 680
FAB99751Y
DyLight 755
FAB99751Z
FITC
FAB99751F
HRP
FAB99751H
Janelia Fluor 549
FAB99751I
Janelia Fluor 646
FAB99751J
PE
FAB99751P
PerCP
FAB99751C
mFluor Violet 450 SE
FAB99751MFV450
mFluor Violet 500 SE
FAB99751MFV500
mFluor Violet 610 SE
FAB99751MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB99751-SP
Carrier Free
IC99751V-100UG
Carrier Free
IC99751U-100UG
Carrier Free
IC99751T-100UG
Carrier Free
IC99751S-100UG
Carrier Free
IC99751R-100UG
Carrier Free
IC99751N-100UG
Carrier Free
IC99751G-100UG
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PEG10 Antibody (981902) [Unconjugated] Summary

Immunogen
Synthetic peptide containing human PEG10
Accession # Q86TG7
Specificity
Detects human PEG10 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PEG10 Antibody (981902) [Unconjugated]

  • EDR
  • embryonal carcinoma differentiation regulated
  • Embryonal carcinoma differentiation-regulated protein
  • HB-1
  • KIAA1051EDR
  • Mammalian retrotransposon-derived protein 2
  • Mar2
  • Mart2
  • MEF3 Like 1
  • MEF3L
  • MEF3L1
  • Myelin expression factor 3-like protein 1
  • paternally expressed 10
  • Paternally expressed gene 10 protein
  • PEG10
  • retrotransposon gag domain containing 3
  • Retrotransposon gag domain-containing protein 3
  • Retrotransposon-derived gag-like polyprotein
  • retrotransposon-derived protein PEG10
  • RGAG3
  • RGAG3MEF3-like protein 1
  • RTL2
  • SIRH1
  • Ty3/Gypsy-like protein

Background

Paternally Expressed Gene-10, or PEG10 (a.k.a Retrotransposon-derived protein PEG10, EDR, MART2, MEF3L1 and RGAG3) is a 708 amino acids (aa) protein encoded by the PEG10 gene. PEG-10 Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1, possibly playing a key role in epithelial-mesenchymal transition (EMT). PEG-10 has been suggested to participate in cell proliferation, differentiation and cell apoptosis processes and identified as a prognostic biomarker for progression of several carcinomas, like breast, lung, liver and pancreatic carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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