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PDLIM4 Antibody

Images

 
Western Blot: PDLIM4 Antibody [NB100-1382] - (1ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
Immunocytochemistry/ Immunofluorescence: PDLIM4 Antibody [NB100-1382] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more
Immunocytochemistry/ Immunofluorescence: PDLIM4 Antibody [NB100-1382] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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PDLIM4 Antibody Summary

Immunogen
Peptide with sequence C-DVVAVYPNAKVELV corresponding to C-Terminus according to NP_003678.2.
Predicted Species
Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDLIM4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1.0 -3.0 ug/ml
Application Notes
Western blot: Approx 37kDa band observed in lysates of cell line A431 (calculated MW of 35.4kDa according to NP_003678.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Control
A-431 Whole Cell Lysate
Rab13 Overexpression Lysate
Publications
Read Publication using NB100-1382.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDLIM4 Antibody

  • LIM domain protein
  • LIM protein RIL
  • PDZ and LIM domain 4
  • PDZ and LIM domain protein 4
  • Reversion-induced LIM protein
  • RILenigma homolog

Background

RIL was originally identified as a gene downregulated in H-ras-transformed cells. RIL was mostly expressed in nonmuscle epithelial cells with a pattern distinct from that of CLP-36. RIL protein was found to localize to actin stress fibers in nonmuscle cells similarly to CLP-36. However, RIL expression led to partially abnormal actin filaments showing thick irregular stress fibers not seen with CLP-36. The RIL PDZ-LIM protein is a regulator of actin stress fiber turnover.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDLIM4 Antibody (NB100-1382)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDLIM4 Antibody (NB100-1382) (0)

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Bioinformatics

Gene Symbol PDLIM4
Entrez
Uniprot