![Western Blot: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Western blot analysis of PDCD6 on different lysates with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lane 3: 293T cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32728) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32728_mouse-pdcd6-mab-a6a7-r-278202415242611.jpg "Western Blot: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Western blot analysis of PDCD6 on different lysates with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lane 3: 293T cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32728) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF |
| Clone | A6A7-R |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within Human PDCD6 aa 1-191 / 191. (Uniprot: O75340) |
| Localization | Endosome, Nucleus, Endoplasmic reticulum membrane, Cytoplasm, COPII-coated vesicle membrane. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PDCD6 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for PDCD6 Antibody (NBP3-32728)Find related products by research area.
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| Gene Symbol | PDCD6 |
![Immunocytochemistry/ Immunofluorescence: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Immunocytochemistry analysis of HeLa cells labeling PDCD6 with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32728_mouse-pdcd6-mab-a6a7-r-292024111480.jpg "Immunocytochemistry/ Immunofluorescence: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Immunocytochemistry analysis of HeLa cells labeling PDCD6 with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PDCD6 antibody (NBP3-32728) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Flow cytometric analysis of HeLa cells labeling PDCD6. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32728, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32728_mouse-pdcd6-mab-a6a7-r-392024884677.jpg "Flow Cytometry: PDCD6 Antibody (A6A7-R) [NBP3-32728] - Flow cytometric analysis of HeLa cells labeling PDCD6. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32728, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")