![ELISA: Human PARP ELISA Kit (Colorimetric) [NBP3-18217] - Standard Curve Reference](http://images.novusbio.com/fullsize/Human-PARP-ELISA-Kit-Colorimetric-ELISA-NBP3-18217-img0001.jpg "ELISA: Human PARP ELISA Kit (Colorimetric) [NBP3-18217] - Standard Curve Reference")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Cell lysates, tissue homogenates and other biological fluids |
| Standard Curve Range | 0.78-50 ng/ml |
| Sensitivity | 0.33 ng/ml |
| Description | The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Human PARP in the sample, this Human PARP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Human PARP concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Standard Curve Range | 0.78-50 ng/ml |
| Sensitivity | 0.33 ng/ml |
| Assay Type | Sandwich ELISA |
| Inter-Assay | %CV < 15 |
| Intra-Assay | %CV < 10 |
| Sample Volume | 100 ul |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | PARP1 |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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| Gene Symbol | PARP1 |
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![Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - (Azide Free), Cleaved [NBP2-27335] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](https://images.novusbio.com/images/PARP-Antibody-194C1439-Azide-Free-Cleaved-Immunocytochemistry-Immunofluorescence-NBP2-27335-img0004.jpg "Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - (Azide Free), Cleaved [NBP2-27335] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")