![Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) - Azide and BSA Free [NBP3-08660] - Formalin-fixed, paraffin-embedded human kidney stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-rTP40-3690-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08660-img0003.jpg "Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) - Azide and BSA Free [NBP3-08660] - Formalin-fixed, paraffin-embedded human kidney stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC |
| Clone | rTP40/3690 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml. of antibody purified by Protein A Column prepared in 10 mM PBS WITHOUT BSA & azide. Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | A synthetic peptide from the N-terminal of human p63/TP73Lprotein (Exact sequence is proprietary) (Uniprot: Q9H3D4 ) |
| Localization | Nuclear |
| Marker | Squamous, Basal & Myoepithelial Cell Marker |
| Specificity | p63 consists of two major isoforms-TAp63 and delta-Np63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation-competent TA domain with homology to p53, which regulates the expression of the growth-inhibitory genes. In contrast, DNp63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally-inactive delta-N domain, which antagonizes the activity of TAp63 and p53. P40/3980R recognizes exclusively delta-Np63 but not TAp63. p40 is a squamous cell carcinoma specific antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma vs. invasive breast ductal carcinoma. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TP63 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for p63/TP73L Antibody (NBP3-08660)Find related products by research area.
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| Gene Symbol | TP63 |
![Flow Cytometry: p63/TP73L Antibody (rTP40/3690) - Azide and BSA Free [NBP3-08660] - Flow Cytometric Analysis of PFA-fixed HeLa cells using p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-rTP40-3690-Azide-and-BSA-Free-Flow-Cytometry-NBP3-08660-img0001.jpg "Flow Cytometry: p63/TP73L Antibody (rTP40/3690) - Azide and BSA Free [NBP3-08660] - Flow Cytometric Analysis of PFA-fixed HeLa cells using p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).")
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