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Recombinant Human p21/CIP1/CDKN1A His Protein

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SDS-Page: Recombinant Human p21/CIP1/CDKN1A Protein [NBP2-22976]
SDS-Page: Recombinant Human p21/CIP1/CDKN1A Protein [NBP2-22976]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human p21/CIP1/CDKN1A His Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-164 of Human p21/CIP1/CDKN1A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSEPAGDVRQ NPCGSKACRR LFGPVDSEQL SRDCDALMAG CIQEARERWN FDFVTETPLE GDFAWERVRG LGLPKLYLPT GPRRGRDELG GGRRPGTSPA LLQGTAEEDH VDLSLSCTLV PRSGEQAEGS PGGPGDSQGR KRRQTSMTDF YHSKRRLIFS KRKP
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
CDKN1A
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Theoretical MW
20.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 10% glycerol, 0.1 M NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human p21/CIP1/CDKN1A His Protein

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1

Background

CDKN1A, also known as P21 or WAF1, plays a critical role in the cellular response to DNA damage, and its overexpression results in cell cycle arrest. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S. However, CDKN1A may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D. This protein may be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Recombinant human CDKN1A protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol CDKN1A