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p21/CIP1/CDKN1A Antibody (DCS-60.2) [CoraFluor™ 1]

 
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Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone
DCS-60.2
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47781AF350
Alexa Fluor 405
NBP2-47781AF405
Alexa Fluor 488
NBP2-47781AF488
Alexa Fluor 532
NBP2-47781AF532
Alexa Fluor 594
NBP2-47781AF594
Alexa Fluor 647
NBP2-47781AF647
Alexa Fluor 700
NBP2-47781AF700
Alexa Fluor 750
NBP2-47781AF750
Allophycocyanin
NBP2-47781APC
Biotin
NBP2-47781B
DyLight 350
NBP2-47781UV
DyLight 405
NBP2-47781V
DyLight 488
NBP2-47781G
DyLight 550
NBP2-47781R
DyLight 594
NBP2-47781DL594
DyLight 650
NBP2-47781C
DyLight 680
NBP2-47781FR
DyLight 755
NBP2-47781IR
FITC
NBP2-47781F
HRP
NBP2-47781H
Janelia Fluor 525
NBP2-47781JF525
Janelia Fluor 549
NBP2-47781JF549
Janelia Fluor 646
NBP2-47781JF646
Janelia Fluor 669
NBP2-47781JF669
PE
NBP2-47781PE
PerCP
NBP2-47781PCP
Unconjugated
NBP2-44525-0.1mg
mFluor Violet 450 SE
NBP2-47781MFV450
mFluor Violet 500 SE
NBP2-47781MFV500
mFluor Violet 610 SE
NBP2-47781MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44525-0.1mg
Azide and BSA Free
NBP2-47781-0.1mg
IHC-Prediluted
NBP2-48351
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p21/CIP1/CDKN1A Antibody (DCS-60.2) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant full-length human p21/CIP1/CDKN1A protein (Uniprot: P38936)
Localization
Nuclear
Specificity
This monoclonal antibody recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This monoclonal antibody is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Mouse or Rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for p21/CIP1/CDKN1A Antibody (DCS-60.2) [CoraFluor™ 1]

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDKN1A