![Flow Cytometry: Oxytocin/Neurophysin I Antibody [NBP2-76346] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Oxytocin-Antibody-Flow-Cytometry-NBP2-76346-img0002.jpg "Flow Cytometry: Oxytocin/Neurophysin I Antibody [NBP2-76346] - Analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Immunogen | Peptide with sequence DGCRTDPACDPES corresponding to C-Terminus (near) according to Uniprot Mouse NP_035155.1. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | OXT |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Preliminary experiments gave an approx. 22 kDa band in mouse liver and lung lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 12.9 kDa band according to NP_035155.1. The 22 kDa band was successfully blocked by incubation with the immunizing peptide. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | OXT |
![Immunofluorescence: Oxytocin/Neurophysin I Antibody [NBP2-76346] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/Oxytocin-Antibody-Immunofluorescence-NBP2-76346-img0001.jpg "Immunofluorescence: Oxytocin/Neurophysin I Antibody [NBP2-76346] - Analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
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