Home » OVGP1 » OVGP1 Lysates » OVGP1 Overexpression Lysate

OVGP1 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: OVGP1 Overexpression Lysate (Adult Normal) [NBP2-08101] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OVGP1.
Western Blot: OVGP1 Overexpression Lysate (Adult Normal) [NBP2-08101] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OVGP1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals offers 100% Guarantee

OVGP1 Overexpression Lysate Summary

Description

OVGP1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC221684

Accession#: NM_002557

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
OVGP1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
75.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for OVGP1 Overexpression Lysate

  • CHIT5
  • EGP
  • Estrogen-dependent oviduct protein
  • MUC9
  • mucin 9
  • mucin-9
  • OGP
  • oviduct glycoprotein
  • oviductal glycoprotein 1, 120kDa
  • Oviductal glycoprotein
  • oviductin
  • oviduct-specific glycoprotein

Background

The OVGP1 gene encodes a large, carbohydrate-rich, epithelial glycoprotein with numerous O-glycosylation sites located within threonine, serine, and proline-rich tandem repeats. The gene is similar to members of the mucin and the glycosyl hydrolase 18 gene fam

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(25 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
G6PC Antibody
(CL5817) - Azide and ...
NBP3-44192
G6PC Antibody (CL5817) - Azide and BSA Free
Species: Hu
Applications: IHC, IHC-P
IGF-I/IGF-1
[Unconjugated]
291-G1
SDS-PAGE IGF-I/IGF-1 [Unconjugated]
Bioactivity IGF-I/IGF-1 [Unconjugated]
Mass Spectrometry IGF-I/IGF-1 [Unconjugated]
(224 Publications)
Species: Hu
Applications: BA
MUC1 Antibody
(SM3) - BSA Free
NB120-22711
Immunocytochemistry/ Immunofluorescence MUC1 Antibody (SM3) - BSA Free
Flow Cytometry MUC1 Antibody (SM3) - BSA Free
Immunocytochemistry/ Immunofluorescence MUC1 Antibody (SM3) - BSA Free
(14 Publications)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Glucagon Antibody
(181402) [Unconjugate ...
MAB1249
Immunocytochemistry Glucagon Antibody (181402) [Unconjugated]
Intracellular Staining by Flow Cytometry Glucagon Antibody (181402) [Unconjugated]
(1 Review)
(15 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
Glucokinase/GCK
Antibody (OTI3E3)
NBP1-47778
Immunocytochemistry/ Immunofluorescence Glucokinase/GCK Antibody (OTI3E3)
Immunohistochemistry-Paraffin Glucokinase/GCK Antibody (OTI3E3)
Immunohistochemistry-Paraffin Glucokinase/GCK Antibody (OTI3E3)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
EpCAM/TROP1
Antibody [Unconjugate
AF960
Flow Cytometry EpCAM/TROP1 Antibody [Unconjugated]
Immunohistochemistry EpCAM/TROP1 Antibody [Unconjugated]
Immunohistochemistry EpCAM/TROP1 Antibody [Unconjugated]
(37 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
ZP3 Antibody - BSA
Free
NBP2-30830
Immunohistochemistry-Paraffin ZP3 Antibody - BSA Free
Immunohistochemistry-Paraffin ZP3 Antibody - BSA Free
Immunohistochemistry-Paraffin ZP3 Antibody - BSA Free
(2 Publications)
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P
CRAT Antibody -
BSA Free
NBP1-86616
Western Blot CRAT Antibody - BSA Free
Western Blot CRAT Antibody - BSA Free
Immunohistochemistry-Paraffin CRAT Antibody - BSA Free
(2 Publications)
Species: Hu
Applications: IHC, IHC-P, WB
GLYAT Antibody -
BSA Free
NBP2-58917
Western Blot GLYAT Antibody - BSA Free
Immunohistochemistry-Paraffin GLYAT Antibody - BSA Free
Immunohistochemistry-Paraffin GLYAT Antibody - BSA Free
Species: Hu
Applications: IHC, IHC-P, WB
Catalase Antibody
[Unconjugated]
AF3398
Western Blot Catalase Antibody [Unconjugated]
Simple Western Catalase Antibody [Unconjugated]
Knockout Validated Catalase Antibody [Unconjugated]
(3 Reviews)
(15 Publications)
Species: Hu, Mu, Rt
Applications: ICC, KO, Simple Western, WB
ER alpha/NR3A1
Antibody (33)
NB300-560
Western Blot ER alpha/NR3A1 Antibody (33)
Immunohistochemistry-Frozen ER alpha/NR3A1 Antibody (33)
Immunohistochemistry-Paraffin ER alpha/NR3A1 Antibody (33)
(1 Review)
(11 Publications)
Species: Av, Bv, Ma, Hu, Mu, Pm, Rt
Applications: IF, IHC, IHC-Fr, IHC-P, WB
MUC6 Antibody
(CLH5)
NBP2-44374
Immunohistochemistry-Paraffin MUC6 Antibody (CLH5)
Immunohistochemistry-Paraffin MUC6 Antibody (CLH5)
(4 Publications)
Species: Hu
Applications: Flow, ICC/IF, IF, IHC, IHC-P, IP, WB
MUC5AC Antibody
(45M1)
NBP2-15196
Immunohistochemistry-Paraffin MUC5AC Antibody (45M1)
Immunohistochemistry-Paraffin MUC5AC Antibody (45M1)
Immunohistochemistry-Paraffin MUC5AC Antibody (45M1)
(5 Reviews)
(28 Publications)
Species: Bv(-), Ch, Fe, Hu, Ma, Pm, Mu, Po, Rb, Rt
Applications: DB, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
Albumin Antibody
(188835) [Unconjugate ...
MAB1455
Simple Western Albumin Antibody (188835) [Unconjugated] - Serum
Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum
Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum
(3 Reviews)
(56 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Growth Hormone
Antibody [Unconjugate
AF1067
Western Blot Growth Hormone Antibody [Unconjugated]
ELISA Growth Hormone Antibody [Unconjugated]
Neutralization Growth Hormone Antibody [Unconjugated]
(15 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, IHC, Neut, WB

Osteoprotegerin/TNFRSF
DY805
N/A Osteoprotegerin/TNFRSF11B [Biotin]
(90 Publications)
Species: Hu
Applications: ELISA
AKT [p Ser473]
Antibody [Unconjugate ...
AF887
Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific
(51 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB

Publications for OVGP1 Lysate (NBP2-08101) (0)

There are no publications for OVGP1 Lysate (NBP2-08101).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for OVGP1 Lysate (NBP2-08101) (0)

There are no reviews for OVGP1 Lysate (NBP2-08101). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for OVGP1 Lysate (NBP2-08101) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional OVGP1 Products

Blogs on OVGP1

There are no specific blogs for OVGP1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our OVGP1 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol OVGP1