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Human Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)

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Human Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) [NBP2-60099] - Standard Curve Reference
Human Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) [NBP2-60099] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and tissue homogenates
Standard Curve Range
7.8 pg/mL - 500 pg/mL
Sensitivity
1.95 pg/mL

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Human Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of human OPG. No significant cross-reactivity or interference between human OPG and analogues was observed.
Standard Curve Range
7.8 pg/mL - 500 pg/mL
Sensitivity
1.95 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFRSF11B

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Human Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)

  • OCIF
  • OCIFMGC29565
  • OPG
  • OPGtumor necrosis factor receptor superfamily member 11B
  • Osteoclastogenesis inhibitory factor
  • Osteoprotegerin
  • TNFRSF11B
  • TR1
  • tumor necrosis factor receptor superfamily, member 11b

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OPG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OPG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OPG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OPG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human Osteoprotegerin/TNFRSF11B ELISA Kit (Colorimetric)
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Bioinformatics

Gene Symbol TNFRSF11B