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Osteoactivin/GPNMB Antibody (297310)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clone
297310
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

  • Size / Catalog#QtyPrice
    MAB2330-SP
    25 ug
    $149.00
    5-7 Business Days
    MAB2330
    100 ug
    $429.00
    1-3 Business Days
    View Available Formulations
    Catalog# & Formulation Size Price
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Support & Research

Osteoactivin/GPNMB Antibody (297310) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Osteoactivin/GPNMB
Lys23-Asn502
Accession # NP_444340
Specificity
Detects mouse Osteoactivin/GPNMB in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human Osteoactivin.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
GPNMB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteoactivin/GPNMB Antibody (297310)

  • DC-HIL
  • glycoprotein (transmembrane) nmb
  • glycoprotein NMB
  • GPNMB
  • HGFIN
  • HGFINglycoprotein nmb-like protein
  • NMBosteoactivin
  • Osteoactivin
  • Transmembrane glycoprotein HGFIN
  • transmembrane glycoprotein NMB

Background

Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.

  1. Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
  2. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
  3. Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GPNMB
Uniprot