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Ornithine Decarboxylase Antibody (ODC1/485) - Azide and BSA Free

Images

 
Western Blot: Ornithine Decarboxylase Antibody (ODC1/485) - Azide and BSA Free [NBP2-34700] - analysis of ODC1 in human placental lysate
Immunocytochemistry/ Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/485) - Azide and BSA Free [NBP2-34700] - IF staining of LNCap cells using AF488 labeled ODC1 Monoclonal Antibody (ODC1/485) (GREEN) DAPI ...read more
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) - Azide and BSA Free [NBP2-34700] - Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, ...read more
Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/485) - Azide and BSA Free [NBP2-34700] - Flow Cytometric Analysis of human ODC-1 in PC3 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ...read more
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) - Azide and BSA Free [NBP2-34700] - Formalin-fixed, paraffin-embedded human prostate carcinoma stained with ODC-1 Monoclonal Antibody (ODC1/485)
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) - Azide and BSA Free [NBP2-34700] - Formalin-fixed paraffin-embedded human testicular carcinoma stained with ODC-1 Monoclonal Antibody (ODC1/485)
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) - Azide and BSA Free [NBP2-34700] - Formalin-fixed. paraffin-embedded rat pancreas stained with ODC1 Monoclonal Antibody (ODC1/485)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
ODC1/485
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

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Ornithine Decarboxylase Antibody (ODC1/485) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32887).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant full-length human Ornithine Decarboxylase protein (Uniprot: P11926)
Localization
Cytoplasmic
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ODC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Western Blot 0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Ornithine Decarboxylase Antibody (ODC1/485) - Azide and BSA Free

  • ODC
  • ODC1
  • ODCEC 4.1.1.17
  • ornithine decarboxylase 1
  • ornithine decarboxylase

Background

Ornithine Decarboxylase encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ODC1