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Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

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ELISA: Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric) [NBP2-80230] - Samples were spiked with high concentrations of Human Orexin A/Hypocretin-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric) [NBP2-80230] - Samples were spiked with high concentrations of Human Orexin A/Hypocretin-1 and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric) [NBP2-80230] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
62.5-4000 pg/mL
Sensitivity
37.5 pg/mL

Order Details

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Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human OXA in samples. No significant cross-reactivity or interference between Human OXA and analogues was observed.
Standard Curve Range
62.5-4000 pg/mL
Sensitivity
37.5 pg/mL
Assay Type
Competitive-ELISA
Inter-Assay
CV% < 4.59%
Intra-Assay
CV% < 4.71%
Spike Recovery
88-111%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
HCRT

Applications/Dilutions

Dilutions
  • ELISA
Reviewed Applications
Read 1 Review rated 5
using
NBP2-80230 in the following application:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25X)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)

  • HCRT
  • Hcrt1
  • hypocretin (orexin) neuropeptide precursor
  • Hypocretin1
  • Hypocretin-1
  • NRCLP1
  • Orexin A
  • orexin
  • OXHcrt
  • PPORX
  • PPOXHypocretin

Background

FUNCTION: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity (By similarity). SUBCELLULAR LOCATION: Endoplasmic reticulum; rough endoplasmic reticulum (By similarity). Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses (By similarity). SIMILARITY: Belongs to the orexin family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human Orexin
A/Hypocretin-1 ELISA ...
NBP2-80230
Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric)
Species: Hu
Applications: ELISA

Publications for Orexin A/Hypocretin-1 ELISA Kit (NBP2-80230) (0)

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Review for Orexin A/Hypocretin-1 ELISA Kit (NBP2-80230) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP2-80230:
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reviewed by:
Anna Picca
 06/10/2022
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Summary

Sample TestedHuman serum
Lot2K1DA8F5E5

Product General Protocols

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Contact Information

Product PDFs

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  Human Orexin A/Hypocretin-1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Recent Reviews

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Orexin A/Hypocretin-1 NBP2-80230
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 Anna Picca
06/10/2022
Application:
Species:
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Bioinformatics

Gene Symbol HCRT
Uniprot