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NUCKS1 Antibody

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Immunohistochemistry: NUCKS1 Antibody [NB100-74634] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NUCKS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Independent Antibodies: Immunoprecipitation: NUCKS1 Antibody [NB100-74634] - Detection of Human NUCKS by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

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NUCKS1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate using the numbering given in entry NP_073568.2 (GeneID 64710).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NUCKS1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 -1:2000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NUCKS1 Antibody

  • FLJ21480
  • FLJ32016
  • FLJ38536
  • NUCKSnuclear ubiquitous casein and cyclin-dependent kinases substrate
  • nuclear casein kinase and cyclin-dependent kinase substrate 1
  • nuclear ubiquitous casein kinase and cyclin-dependent kinase substrate
  • P1
  • potential LAG1 interactor

Background

NUCKS (nuclear ubiquitous casein and cyclin-dependent kinases substrate) is a nuclear phosphoprotein phosphorylated by Cdk1 during mitosis. It is a DNA-binding protein and bears two putative nuclear localization signals. NUCKS has been shown to localize to the cytoplasm in mitotic cells and is targeted to the nucleus during telophase. It is expressed ubiquitously and possesses features of a housekeeping gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NUCKS1
Entrez
Uniprot