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NQO-2 Antibody

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Western Blot: NQO-2 Antibody [NBP1-85221] - Analysis in control (vector only transfected HEK293T lysate) and NQO2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: NQO-2 Antibody [NBP1-85221] - Staining of human cell line U-251 MG shows localization to nucleoplasm and cytosol.
Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85221] - Staining of human urinary bladder shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85221] - Analysis in human kidney and urinary bladder tissues. Corresponding NQO2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85221] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: NQO-2 Antibody [NBP1-85221] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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NQO-2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNLEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NQO2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000-1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NQO-2 Protein (NBP1-85221PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NQO-2 Antibody

  • DHQV
  • DIA6
  • dioxin-inducible-2
  • EC 1.10.99.2
  • NAD(P)H dehydrogenase, quinone 2
  • NAD(P)H menadione oxidoreductase 2, dioxin-inducible
  • NMOR2
  • NQO2
  • NQO-2
  • NRH dehydrogenase [quinone] 2
  • NRH:quinone oxidoreductase 2
  • QR2
  • Quinone Reductase 2
  • ribosyldihydronicotinamide dehydrogenase [quinone]
  • ribosyldihydronicotinamide dehydrogenase

Background

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NQO2