NPLOC4 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82165] - Staining in human testis and pancreas tissues using anti-NPLOC4 antibody. Corresponding NPLOC4 RNA-seq data are presented for ...read more
Independent Antibodies: Western Blot: NPLOC4 Antibody [NBP1-82165] - Analysis using Anti-NPLOC4 antibody NBP1-82165 (A) shows similar pattern to independent antibody NBP1-82166 (B).
Immunocytochemistry/ Immunofluorescence: NPLOC4 Antibody [NBP1-82165] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82165] - Staining of human pancreas shows low expression as expected.
Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82165] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NPLOC4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYL
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPLOC4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NPLOC4 Protein (NBP1-82165PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NPLOC4 Antibody

  • FLJ20657
  • FLJ23742
  • KIAA1499nuclear protein localization protein 4 homolog
  • NPL4Protein NPL4
  • nuclear protein localization 4 homolog (S. cerevisiae)

Background

The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NPLOC4