![ELISA: Human non-muscle Myosin IIA ELISA Kit (Colorimetric) [NBP3-39552] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39552_human-non-muscle-myosin-iia-elisa-kit-colorimetric-2110202415355079.png "ELISA: Human non-muscle Myosin IIA ELISA Kit (Colorimetric) [NBP3-39552] - Standard Curve Reference")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Serum, plasma and other biological fluids |
| Standard Curve Range | 0.156 - 10 ng/mL (example only; lot dependent) |
| Sensitivity | 0.063 ng/mL (example only; lot dependent) |
| Description | Assay Length: 4.5 hours |
| Standard Curve Range | 0.156 - 10 ng/mL (example only; lot dependent) |
| Sensitivity | 0.063 ng/mL (example only; lot dependent) |
| Assay Type | Sandwich ELISA |
| Inter-Assay | %CV < 12 (example only; lot dependent) |
| Intra-Assay | %CV < 10 (example only; lot dependent) |
| Sample Volume | 100 uL |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | MYH9 |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
Research Areas for non-muscle Myosin IIA ELISA Kit (NBP3-39552)Find related products by research area.
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| Gene Symbol | MYH9 |
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![Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Bovine ovarian granulosa cells. Anti Myosin IIA immunofluorescent staining (green). DNA in counterstained with DAPI (blue). Control was obtained by omitting the primary antibody. Fixation: 4% PF for 7 minutes at rt. Permeabilization: 0.1% Triton-X in PBS for 7 minutes. Blocking: 20% donkey serum, 1% BSA in PBS, 1 hour at rt. Primary antibody: 1:50 or 1:100 in 1% BSA/PBS over night at 4C. Secondary antibody: donkey anti mouse -Alexa 488 (Invitrogen), 1:500. DNA Counterstaining: mounting medium with DAPI. Negative CTRL was performed by omitting the primary antibody, images were captured under an epifluorescence microscope. Image from verified customer review.](https://images.novusbio.com/images/non-muscle-Myosin-IIA-Antibody-2B3-Immunocytochemistry-Immunofluorescence-H00004627-M03-img0007.jpg "Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Bovine ovarian granulosa cells. Anti Myosin IIA immunofluorescent staining (green). DNA in counterstained with DAPI (blue). Control was obtained by omitting the primary antibody. Fixation: 4% PF for 7 minutes at rt. Permeabilization: 0.1% Triton-X in PBS for 7 minutes. Blocking: 20% donkey serum, 1% BSA in PBS, 1 hour at rt. Primary antibody: 1:50 or 1:100 in 1% BSA/PBS over night at 4C. Secondary antibody: donkey anti mouse -Alexa 488 (Invitrogen), 1:500. DNA Counterstaining: mounting medium with DAPI. Negative CTRL was performed by omitting the primary antibody, images were captured under an epifluorescence microscope. Image from verified customer review.")