![Western Blot: non-muscle Myosin IIA Antibody (GT566) [NBP3-13580] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [GT566] (NBP3-13580) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-GT566-Western-Blot-NBP3-13580-img0005.jpg "Western Blot: non-muscle Myosin IIA Antibody (GT566) [NBP3-13580] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [GT566] (NBP3-13580) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, CaSpecies Glossary |
| Applications | WB, IHC |
| Clone | GT566 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human non-muscle Myosin IIA. The exact sequence is proprietary. |
| Predicted Species | Rat (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MYH9 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 227 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for non-muscle Myosin IIA Antibody (NBP3-13580)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MYH9 |
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT566) [NBP3-13580] - non-muscle Myosin IIA antibody [GT566] detects non-muscle Myosin IIA protein at cytoplasm in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon. non-muscle Myosin IIA antibody [GT566] (NBP3-13580) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-GT566-Immunohistochemistry-Paraffin-NBP3-13580-img0004.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT566) [NBP3-13580] - non-muscle Myosin IIA antibody [GT566] detects non-muscle Myosin IIA protein at cytoplasm in human colon by immunohistochemical analysis. Sample: Paraffin-embedded human colon. non-muscle Myosin IIA antibody [GT566] (NBP3-13580) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: non-muscle Myosin IIA Antibody (GT566) [NBP3-13580] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [GT566] (NBP3-13580) diluted at 1:1000.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-GT566-Western-Blot-NBP3-13580-img0001.jpg "Western Blot: non-muscle Myosin IIA Antibody (GT566) [NBP3-13580] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [GT566] (NBP3-13580) diluted at 1:1000.")
![Western Blot: non-muscle Myosin IIA Antibody (GT566) [NBP3-13580] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [GT566] (NBP3-13580) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-GT566-Western-Blot-NBP3-13580-img0002.jpg "Western Blot: non-muscle Myosin IIA Antibody (GT566) [NBP3-13580] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with non-muscle Myosin IIA antibody [GT566] (NBP3-13580) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT566) [NBP3-13580] - non-muscle Myosin IIA antibody [GT566] detects non-muscle Myosin IIA protein at cytoplasm in human mammary gland by immunohistochemical analysis. Sample: Paraffin-embedded human mammary gland. non-muscle Myosin IIA antibody [GT566] (NBP3-13580) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-GT566-Immunohistochemistry-Paraffin-NBP3-13580-img0003.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (GT566) [NBP3-13580] - non-muscle Myosin IIA antibody [GT566] detects non-muscle Myosin IIA protein at cytoplasm in human mammary gland by immunohistochemical analysis. Sample: Paraffin-embedded human mammary gland. non-muscle Myosin IIA antibody [GT566] (NBP3-13580) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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