![Western Blot: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neurogenin-3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Neurogenin-3.](http://images.novusbio.com/fullsize/Neurogenin-3-Antibody-OTI3B5-Western-Blot-NBP1-47878-img0007.jpg "Western Blot: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neurogenin-3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Neurogenin-3.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | OTI3B5 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Full-length protein expressed in 293T cell transfected with human NEUROG3 expression vector |
| Specificity | This antibody is specific for Homo sapiens neurogenin 3 (NEUROG3). |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NEUROG3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 23.077 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - Neurogenin3 Immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY Neurogenin-3.](http://images.novusbio.com/fullsize/Neurogenin-3-Antibody-OTI3B5-Immunocytochemistry-Immunofluorescence-NBP1-47878-img0005.jpg "Immunocytochemistry/Immunofluorescence: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - Neurogenin3 Immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY Neurogenin-3.")
![Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/Neurogenin-3-Antibody-OTI3B5-Immunohistochemistry-Paraffin-NBP1-47878-img0008.jpg "Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody at 1:150 dilution.")
![Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - Staining of paraffin-embedded Human lymph node tissue using anti-Neurogenin-3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Neurogenin-3-Antibody-OTI3B5-Immunohistochemistry-Paraffin-NBP1-47878-img0006.jpg "Immunohistochemistry-Paraffin: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - Staining of paraffin-embedded Human lymph node tissue using anti-Neurogenin-3 mouse monoclonal antibody.")
![Immunoprecipitation: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - (Negative control: IP without adding anti-NEUROG3 antibody). For each experiment, 500ul of DDK tagged NEUROG3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/Neurogenin-3-Antibody-OTI3B5-Immunoprecipitation-NBP1-47878-img0009.jpg "Immunoprecipitation: Neurogenin-3 Antibody (OTI3B5) [NBP1-47878] - (Negative control: IP without adding anti-NEUROG3 antibody). For each experiment, 500ul of DDK tagged NEUROG3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.")
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