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Nesfatin-1/Nucleobindin-2 Antibody (877140) [Unconjugated]

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Western blot shows lysates of AGS human gastric adenocarcinoma cell line and human testis tissue. PVDF membrane was probed with 3 µg/mL of Rat Anti-Human Nesfatin-1/Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Nesfatin-1/ Nucleobindin-2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of AGS human gastric adenocarcinoma cell line and human testis tissue. PVDF membrane was probed with 3 µg/mL of Rat Anti-Human Nesfatin-1/Nucleobindin-2 Monoclonal Antibody (Catalog # ...read more
Nesfatin-1/Nucleobindin-2 was detected in immersion fixed human mesenchymal stem cells differentiated into adipocytes using Rat Anti-Human Nesfatin-1/Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) at 10 µg/mL ...read more
Nesfatin-1/Nucleobindin-2 was detected in immersion fixed paraffin-embedded sections of human stomach using Rat Anti-Human Nesfatin-1/ Nucleobindin-2 Monoclonal Antibody (Catalog # MAB5949) at 5 µg/mL overnight at 4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
877140
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5949-SP
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Nesfatin-1/Nucleobindin-2 Antibody (877140) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Nesfatin-1/Nucleobindin-2
Val25-Leu106
Accession # P80303
Specificity
Detects human Nesfatin-1/Nucleobindin-2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
NUCB2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 3 ug/mL
Publications
Read Publications using
MAB5949 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nesfatin-1/Nucleobindin-2 Antibody (877140) [Unconjugated]

  • DNA-binding protein NEFA
  • NEFA
  • NEFAGastric cancer antigen Zg4
  • Nesfatin1
  • Nesfatin-1
  • NUCB2
  • nucleobindin 2
  • Nucleobindin-2
  • nucleobinding 2

Background

Nesfatin-1 (NEFA Encoded Satiety and Fat-influencing protein 1) is a secreted, 10 kDa peptide derived from the translation product of the NUCB2 gene. Nesfatin-1 is associated with neurons involved in feeding (ARH and PVH) and fluid intake (SON and PVH), in beta cells of the pancreas, and in adipocytes. Its presence peripherally has an anorexigenic effect. Mature human Nesfatin-1 is 82 amino acids (aa) in length. It is the secreted N-terminus of a larger, intracellular 420 aa precursor termed NEFA/Nucleobindin-2/NUCB2. NUCB2 contains a signal sequence (aa 1‑24), Nesfatin-1 (aa 25‑106), a DNA-binding site (aa 171‑223), and two EF-hand regions (aa 241‑276 and 293‑328). Intracellular uncleaved NUCB2 is 45‑50 kDa in size. There is a potential for multiple cleavages, generating Nesfatin-2 (aa 109‑187) and Nesfatin-3 (aa 190‑420). Human Nesfatin-1 shares 85% aa identity with mouse Nesfatin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Nesfatin-1/Nucleobindi ...
MAB5949
Nesfatin-1/Nucleobindin-2 Antibody (877140) [Unconjugated]
Species: Hu
Applications: WB, IHC, ICC/IF

Publications for Nesfatin-1/Nucleobindin-2 Antibody (MAB5949)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


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Bioinformatics

Gene Symbol NUCB2
Uniprot