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NDUFC2 Antibody

Images

 
Western Blot: NDUFC2 Antibody [NBP1-88934] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: CACO-2
Immunohistochemistry: NDUFC2 Antibody [NBP1-88934] - Staining of human kidney shows strong cytoplasmic positivity in cells of tubules.
Simple Western: NDUFC2 Antibody [NBP1-88934] - Simple Western lane view shows a specific band for NDUFC2 in 0.2 mg/ml of HT-29 (left) and HCT 116 (right) lysate(s). This experiment was performed under reducing ...read more
Simple Western: NDUFC2 Antibody [NBP1-88934] - Electropherogram image of the corresponding Simple Western lane view. NDUFC2 antibody was used at 1:10 dilution on HT-29 and HCT 116 lysate(s) respectively.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NDUFC2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MIARRNPEPLRFLPDEARSLPPPKLTDPRLLYIGFLGYCSGLIDNLIRRRP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NDUFC2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Simple Western 1:10
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Control Peptide
NDUFC2 Protein (NBP1-88934PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NDUFC2 Antibody

  • B14.5b
  • CI-B14.5b
  • complex I subunit B14.5b
  • Complex I-B14.5b
  • HLC-1NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2 (14.5kD, B14.5b)
  • Human lung cancer oncogene 1 protein
  • NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa
  • NADH dehydrogenase [ubiquinone] 1 subunit C2
  • NADHDH2
  • NADH-ubiquinone oxidoreductase subunit B14.5b

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NDUFC2