![Western Blot: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of MTM1 expression in transfected 293T cell line by MTM1 monoclonal antibody (M01), clone 1C10.Lane 1: MTM1 transfected lysate(69.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Myotubularin-Antibody-1C10-Western-Blot-H00004534-M01-img0009.jpg "Western Blot: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of MTM1 expression in transfected 293T cell line by MTM1 monoclonal antibody (M01), clone 1C10.Lane 1: MTM1 transfected lysate(69.9 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, KD |
| Clone | 1C10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | MTM1 (AAH30779, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASASTSKYNSHSLENESIKRTSRDGVNRDLTEAVPRLPGETLITDKEVIYICPFNGPIKGRVYITNYRLYLRSLETDSSLILDVPLGVISRIEKMGGAT |
| Specificity | MTM1 - myotubularin 1 |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MTM1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of monoclonal antibody to MTM1 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Myotubularin-Antibody-1C10-Immunohistochemistry-Paraffin-H00004534-M01-img0006.jpg "Immunohistochemistry-Paraffin: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of monoclonal antibody to MTM1 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.")
![Western Blot: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of MTM1 over-expressed 293 cell line, cotransfected with MTM1 Validated Chimera RNAi ( Cat # H00004534-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MTM1 monoclonal antibody (M01), clone 1C10 (Cat # H00004534-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Myotubularin-Antibody-1C10-Western-Blot-H00004534-M01-img0007.jpg "Western Blot: Myotubularin Antibody (1C10) [H00004534-M01] - Analysis of MTM1 over-expressed 293 cell line, cotransfected with MTM1 Validated Chimera RNAi ( Cat # H00004534-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MTM1 monoclonal antibody (M01), clone 1C10 (Cat # H00004534-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Sandwich ELISA: Myotubularin Antibody (1C10) [H00004534-M01] - Detection limit for recombinant GST tagged MTM1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Myotubularin-Antibody-1C10-Sandwich-ELISA-H00004534-M01-img0008.jpg "Sandwich ELISA: Myotubularin Antibody (1C10) [H00004534-M01] - Detection limit for recombinant GST tagged MTM1 is approximately 1ng/ml as a capture antibody.")
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