![Western Blot: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC expression in transfected 293T cell line by MYOC monoclonal antibody (M01), clone 4F8.Lane 1: MYOC transfected lysate(57 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Myocilin-Antibody-4F8-Western-Blot-H00004653-M01-img0010.jpg "Western Blot: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC expression in transfected 293T cell line by MYOC monoclonal antibody (M01), clone 4F8.Lane 1: MYOC transfected lysate(57 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, IHC, IP, KD |
| Clone | 4F8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | MYOC (AAH29261, 36 a.a. ~ 135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QLRKANDQSGRCQYTFSVASPNESSCPEQSQAMSVIHNLQRDSSTQRLDLEATKARLSSLESLLHQLTLDQAARPQETQEGLQRELGTLRRERDQLETQT |
| Specificity | MYOC - myocilin, trabecular meshwork inducible glucocorticoid response |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MYOC |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
ICC | Pig | 12/05/2016 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Myocilin Antibody (H00004653-M01)Find related products by research area.
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![Immunohistochemistry-Paraffin: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of monoclonal antibody to MYOC on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Myocilin-Antibody-4F8-Immunohistochemistry-Paraffin-H00004653-M01-img0006.jpg "Immunohistochemistry-Paraffin: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of monoclonal antibody to MYOC on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.")
![Western Blot: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC over-expressed 293 cell line, cotransfected with MYOC Validated Chimera RNAi ( Cat # H00004653-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYOC monoclonal antibody (M01), clone 4F8 (Cat # H00004653-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Myocilin-Antibody-4F8-Western-Blot-H00004653-M01-img0008.jpg "Western Blot: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC over-expressed 293 cell line, cotransfected with MYOC Validated Chimera RNAi ( Cat # H00004653-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYOC monoclonal antibody (M01), clone 4F8 (Cat # H00004653-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.")
![Immunoprecipitation: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC transfected lysate using anti-MYOC monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYOC MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Myocilin-Antibody-4F8-Immunoprecipitation-H00004653-M01-img0007.jpg "Immunoprecipitation: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC transfected lysate using anti-MYOC monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYOC MaxPab rabbit polyclonal antibody.")
![ELISA: Myocilin Antibody (4F8) [H00004653-M01] - Detection limit for recombinant GST tagged MYOC is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Myocilin-Antibody-4F8-ELISA-H00004653-M01-img0011.jpg "ELISA: Myocilin Antibody (4F8) [H00004653-M01] - Detection limit for recombinant GST tagged MYOC is approximately 0.3ng/ml as a capture antibody.")
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