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MT-ND1 Antibody (JE34-93)

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Western Blot: MT-ND1 Antibody (JE34-93) [NBP3-32613] - Western blot analysis of MT-ND1 on different lysates with Rabbit anti-MT-ND1 antibody (NBP3-32613) at 1/1,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
JE34-93
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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MT-ND1 Antibody (JE34-93) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Synthetic peptide within Human MT-ND1 aa 23-67 / 318. (Uniprot: P03886)
Localization
Mitochondrion inner membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ND1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for MT-ND1 Antibody (JE34-93)

  • EC 1.6.5.3
  • mitochondrially encoded NADH dehydrogenase 1
  • MTND1
  • NAD1
  • NADH dehydrogenase subunit 1
  • NADH dehydrogenase, subunit 1 (complex I)
  • NADH1
  • ND1NADH dehydrogenase 1

Background

NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Human complex I is important for energy metabolism because its main function is to transport electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits. A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7),while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 1 (ND1) binds rotenone and rotenone analogs and might be involved in electron transfer to ubiquinone. Mutations in the ND1 gene may be implicated in several disorders, including Leber hereditary optic neuropathy, Alzheimer's disease and Parkinson disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ND1