MSS4 Recombinant Protein Antigen

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

MSS4 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human PIP5K1B.

Source: E. coli

Amino Acid Sequence: YFDTETYNALMKTLQRDCRVLESFKIMDYSLLLGIHFLDHSLKEKEEETPQNVPDAKRTGMQKVLYSTAMESIQGPGKSGDGIITENPDTMGGIPAKSH

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
PIP5K1B
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-87832.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for MSS4 Recombinant Protein Antigen

  • EC 2.7.1
  • MSS4Protein STM-7
  • Phosphatidylinositol-4-phosphate 5-kinase type I beta
  • phosphatidylinositol-4-phosphate 5-kinase type-1 beta
  • phosphatidylinositol-4-phosphate 5-kinase, type I, beta
  • PIP5K1-beta
  • PIP5KIbeta
  • PtdIns(4)P-5-kinase 1 beta
  • STM7EC 2.7.1.68
  • Type I phosphatidylinositol-4-phosphate 5-kinase beta

Background

MSS4, also known by its gene name PIP5K1B, has a 543 amino acid long isoform that is 61 kDa and a 549 amino acid isoform that is approximately 62 kDa. MSS4 plays a role in platelet adhesion, actin filament organization and the biosynthesis of PIP2. Current research on MSS4 is being conducted in relation to several diseases and disorders including kidney disease, schizophrenia, neuronitis and Friedreich's ataxia. MSS4 has also been shown to have interactions with CLIC1, BTK, MCRS1 and PIP5KL1 in pathways such as the RAC1 signaling pathway, CD28 signaling, Phosphatidylinositol signaling and the metabolism of lipids and lipoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PIP5K1B