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MSH2 Antibody (MSH2/2622) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
MSH2/2622
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

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MSH2 Antibody (MSH2/2622) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant fragment (around aa 327-427) of human MSH2 protein (exact sequence is proprietary) (Uniprot: IDP43246)
Localization
Nuclear
Marker
DNA Mismatch Repair Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MSH2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for MSH2 Antibody (MSH2/2622) [CoraFluor™ 1]

  • COCA1
  • DNA mismatch repair protein Msh2
  • FCC1
  • hMSH2
  • HNPCC
  • HNPCC1
  • HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1)
  • LCFS2
  • MSH2
  • mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
  • MutS protein homolog 2

Background

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MSH2 Antibody (NBP3-08957CL1) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MSH2