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MSH2 Antibody (CL13713)

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Western blot analysis in human cell line NTERA-2.
Western blot analysis in human cell line NTERA-2.
Orthogonal Strategies: Analysis in human testis and liver tissues using NBP3-24572 antibody. Corresponding MSH2 RNA-seq data are presented for the same tissues.
Staining of human lymph node shows strong nuclear positivity in germinal center cells.
Staining of human testis cancer (seminoma) shows strong nuclear positivity in tumor cells.
Staining in NTERA-2 cell line with Anti-MSH2 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.
Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong nuclear positivity in tumor cells.
Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.
Staining of human liver shows no nuclear positivity in hepatocytes as expected.
Staining of human small intestine shows strong nuclear positivity in glandular cells.
Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL13713
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

Order Details

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MSH2 Antibody (CL13713) Summary

Immunogen
This antibody was designed to target the recombinant protein MSH2, with the precise immunogen sequence being withheld for proprietary reasons.
Predicted Species
Mouse (95%), Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MSH2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for MSH2 Antibody (CL13713)

  • COCA1
  • DNA mismatch repair protein Msh2
  • FCC1
  • hMSH2
  • HNPCC
  • HNPCC1
  • HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1)
  • LCFS2
  • MSH2
  • mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
  • MutS protein homolog 2

Background

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol MSH2