I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.

Name: Human Moesin - Ready-To-Use ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-39494

Question

Accepted Answer

The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining RNA interference. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	ELISA
Suitable Sample Type	Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range	0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity	0.064 ng/mL (example only; lot dependent)

Description	The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time. Assay Length: 3 hours
Standard Curve Range	0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity	0.064 ng/mL (example only; lot dependent)
Assay Type	Sandwich ELISA
Inter-Assay	%CV < 12 (example only; lot dependent)
Intra-Assay	%CV < 10 (example only; lot dependent)
Sample Volume	100 uL
Kit Type	ELISA Kit (Colorimetric)
Gene	MSN

Human Moesin - Ready-To-Use ELISA Kit (Colorimetric)

Species Glossary

Images

Product Details

Order Details

Human Moesin - Ready-To-Use ELISA Kit (Colorimetric) Summary

Applications/Dilutions

Packaging, Storage & Formulations

Kit Components

Alternate Names for Human Moesin - Ready-To-Use ELISA Kit (Colorimetric)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Moesin ELISA Kit (NBP3-39494) (0)

Reviews for Moesin ELISA Kit (NBP3-39494) (0)

Product General Protocols

FAQs for Moesin ELISA Kit (NBP3-39494). (Showing 1 - 1 of 1 FAQ).

Additional Moesin Products

Research Areas for Moesin ELISA Kit (NBP3-39494)

Blogs on Moesin

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Bioinformatics